Description

Desthiobiotin n-Hydroxysuccinimidyl Ester is a high-purity, activated ester derivative of desthiobiotin, designed for efficient bioconjugation and labeling applications. This compound is a critical reagent for researchers and manufacturers requiring reliable, site-specific modification of biomolecules, particularly in the development of advanced diagnostic and therapeutic platforms. It is essential for professionals in biopharmaceutical R&D, proteomics, and diagnostic assay development who demand consistent performance and high coupling efficiency.

Application

• Bioconjugation Reagent: For the covalent attachment of desthiobiotin tags to proteins, antibodies, peptides, and other biomolecules via primary amines.
• Streptavidin/Biotin Technology: A key component in affinity purification, pull-down assays, and immobilization techniques utilizing the high-affinity desthiobiotin-streptavidin interaction.
• Diagnostic Assay Development: Used to label detection antibodies or antigens in ELISA, lateral flow assays, and other immunoassay formats.
• Proteomics and Protein Analysis: Facilitates the study of protein-protein interactions, cellular localization, and protein function through targeted labeling.
• Drug Delivery & Targeting: Enables the biotinylation of drug carriers, nanoparticles, or therapeutic agents for targeted delivery systems.
• Surface Functionalization: For modifying biosensor surfaces, microarray slides, or chromatographic media with desthiobiotin ligands.

Basic Information

Product Name	Desthiobiotin n-Hydroxysuccinimidyl Ester
CAS No.	80750-24-9
Molecular Formula	C14H21N3O5
Molecular Weight	311.34 g/mol
Synonyms	Desthiobiotin NHS Ester; N-Hydroxysuccinimide Desthiobiotinate; (+)-Desthiobiotin N-Succinimidyl Ester; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic Acid N-Hydroxysuccinimide Ester (desthiobiotin analog); Biotinylation Reagent (desthiobiotin); NHS-Desthiobiotin
EINECS	Contact for details

Quality Control

Our Desthiobiotin n-Hydroxysuccinimidyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis of the active ester group. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.