Description

3-Keto-4-Pentenoyl-Coenzyme A is a key biochemical intermediate in metabolic pathways, particularly in the catabolism of certain amino acids and fatty acids. Its precise structure makes it an essential tool compound for researchers studying enzymatic mechanisms, metabolic flux, and cellular energy regulation. This high-purity biochemical is primarily utilized by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers for advanced metabolic studies and assay development.

Application

• Enzymology Research: Serves as a specific substrate for studying the activity and kinetics of enzymes like 3-ketoacyl-CoA thiolase in the β-oxidation pathway.
• Metabolic Pathway Analysis: Used in in vitro and cell-based assays to trace and quantify metabolic intermediates in leucine and isoleucine degradation.
• Pharmaceutical Development: Employed in target validation and inhibitor screening for metabolic disorders, cancers, or infectious diseases where related pathways are dysregulated.
• Diagnostic Reagent Formulation: A critical component in the development of clinical diagnostic kits for inborn errors of metabolism, such as maple syrup urine disease.
• Biochemical Standard: Acts as a certified reference material (CRM) for the calibration of analytical instruments like HPLC-MS in metabolomics studies.
• Nutritional Science Research: Investigated for its role in energy metabolism and its potential implications in metabolic health and disease states.

Basic Information

Product Name	3-Keto-4-Pentenoyl-Coenzyme A
CAS No.	80724-14-7
Molecular Formula	C26H40N7O19P3S
Molecular Weight	879.61 g/mol
Synonyms	3-Keto-4-pentenoyl-CoA; 3-Oxo-4-pentenoyl-coenzyme A; (S)-3-Oxo-4-pentenoyl-CoA; 4-Pentenoyl-CoA, 3-oxo-; 3-Ketopentenoyl-CoA; 3-Oxopentenoyl-Coenzyme A; β-keto-4-pentenoyl-CoA; 3-Keto-4-pentenoyl-Coenzyme A lithium salt (common form)
EINECS	Contact for details

Quality Control

Our 3-Keto-4-Pentenoyl-Coenzyme A is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and enzymatic activity assays to ensure functional integrity. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and specific biological activity where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Biological Activity (Enzymatic Assay)	≥ 90% of theoretical activity
Solubility	Soluble in water or appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.