Description

N(1)-Cholesterylcarbamoyl-N(8)-(4-Nitrobenzo-2-Oxa-1,3-Diazole)-3,6-Dioxaoctyl-1,8-Diamine is a sophisticated, fluorescently labeled cholesterol derivative designed for advanced research and diagnostic applications. This compound matters because it combines the biological relevance of cholesterol with a highly sensitive NBD (7-nitrobenz-2-oxa-1,3-diazole) fluorophore, enabling precise tracking and visualization in complex systems. It is primarily needed by researchers and developers in lipidomics, membrane biophysics, and cellular imaging, as well as by diagnostic kit manufacturers for developing assays targeting cholesterol transport and metabolism.

Application

• Fluorescent Labeling of Lipids: Used as a vital tool for tagging and visualizing cholesterol and its analogs in biological membranes and lipoproteins.
• Membrane Dynamics & Biophysics Research: Enables the study of lipid raft formation, membrane fluidity, and cholesterol trafficking in live cells.
• Diagnostic Assay Development: Serves as a key component in the development of fluorescence-based diagnostic kits for disorders related to cholesterol metabolism.
• Drug Delivery System Research: Utilized to trace and evaluate the efficiency of liposomal and nanoparticle-based drug delivery carriers.
• High-Throughput Screening (HTS): Acts as a fluorescent probe in assays designed to screen for compounds that modulate cholesterol homeostasis.
• Academic & Pharmaceutical R&D: Fundamental research into atherosclerosis, neurodegenerative diseases, and other conditions linked to lipid dysregulation.

Basic Information

Product Name	N(1)-Cholesterylcarbamoyl-N(8)-(4-Nitrobenzo-2-Oxa-1,3-Diazole)-3,6-Dioxaoctyl-1,8-Diamine
CAS No.	80685-24-1
Molecular Formula	C55H84N6O6
Molecular Weight	925.31 g/mol
Synonyms	NBD-Cholesterol; NBD-CE; 22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3β-yl carbamate; N-Cholesteryl-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-3,6-dioxaoctane-1,8-diamine; Cholesterol-NBD; Fluorescent Cholesterol Derivative; NBD labeled Cholesterol; 3β-[N-(N'-NBD-3,6-dioxaoctyl)carbamoyl]cholest-5-ene
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Every batch undergoes comprehensive analytical characterization, including HPLC for purity verification and spectroscopic methods (NMR, MS) for structural confirmation. We provide a detailed Certificate of Analysis (COA) with each shipment, ensuring traceability and compliance with research-grade specifications. For custom purity grades or bulk quantities, dedicated QC protocols are established to meet your specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Absorption (λmax)	~465 nm (in suitable solvent)
Emission (λmax)	~535 nm (in suitable solvent)
Solubility	Soluble in DMSO, DMF, chloroform; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.