Description

Bilirubin Oxidase CAS NO 80619-01-8 is a highly specific oxidoreductase enzyme that catalyzes the oxidation of bilirubin to biliverdin. This enzymatic activity is critical for precise biochemical analysis and research into metabolic pathways. It is an essential reagent for diagnostic manufacturers, clinical research laboratories, and biotech companies developing assays for liver function and bilirubin metabolism.

Application

• Clinical Diagnostics: Core component in enzymatic colorimetric and electrochemical assays for the quantitative measurement of total and direct bilirubin in serum, plasma, and urine, used in automated clinical chemistry analyzers.
• Biomedical Research: Used as a tool enzyme in studies of heme catabolism, oxidative stress, jaundice, and neonatal hyperbilirubinemia to understand underlying biochemical mechanisms.
• Biosensor Development: Integrated into amperometric and optical biosensor platforms for the rapid, point-of-care detection of bilirubin, enabling real-time monitoring.
• Reagent Manufacturing: Sourced by in-vitro diagnostic (IVD) kit producers for formulating stable, lyophilized, or liquid reagent systems.
• Enzyme Characterization Studies: Employed in biochemical research to study enzyme kinetics, inhibitor effects, and stability under various conditions.
• Potential Biocatalytic Applications: Investigated for use in specialized biocatalysis and bioremediation processes involving the breakdown of tetrapyrroles.

Basic Information

Product Name	Bilirubin Oxidase
CAS No.	80619-01-8
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 52-66 kDa (Source-dependent)
Synonyms	Bilirubin:oxygen oxidoreductase; BOx; BOD; Myrothecium verrucaria Bilirubin Oxidase; EC 1.3.3.5; Bilirubin Oxidase (Myrothecium); Biliverdin-producing oxidase
EINECS	Contact for details

Quality Control

Our Bilirubin Oxidase is produced and tested under a stringent quality management system. Each lot is characterized for critical parameters including specific activity, purity (via SDS-PAGE), and functional performance in standard assay conditions. We provide comprehensive Certificates of Analysis (COA) detailing activity units/mg protein, pH optimum, stability data, and absence of significant interfering activities. Production can adhere to relevant ISO 13485 standards for diagnostic reagent manufacture.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted enzyme solutions should be aliquoted and stored at -20°C to -80°C, avoiding repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Conforms (Oxidizes Bilirubin)
Specific Activity	≥ 10 U/mg protein (Typical)
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	≥ 90% of dry weight
pH Optimum	7.5 - 8.5
Stability	≥ 12 months at -20°C (lyophilized)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.