Description

8-(2,2,6,6-Tetramethylpiperidin-4-Yl-1-Oxyl)Amino-Adenosine Triphosphate is a spin-labeled derivative of adenosine triphosphate (ATP), a critical nucleotide for cellular energy transfer. This compound is specifically engineered for advanced research applications, where the stable nitroxide radical (TEMPO) moiety enables the study of biomolecular dynamics and interactions using techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is an essential tool for biochemists and molecular biologists investigating enzyme kinetics, protein conformational changes, and nucleotide-binding mechanisms in model systems.

Application

• EPR/ESR Spectroscopy: Acts as a spin probe for studying the structure, dynamics, and binding sites of ATP-dependent enzymes and proteins.
• Enzyme Mechanism Studies: Used to investigate the kinetics and conformational changes associated with kinases, ATPases, and other ATP-utilizing enzymes.
• Membrane Protein Research: Employed in probing the function and environment of membrane-bound transporters and channels that interact with ATP.
• Nucleic Acid Research: Useful for exploring the interactions between ATP analogs and nucleic acid structures or processing enzymes.
• Biophysical Labeling: Serves as a site-directed spin label to monitor distance changes and molecular motions within large protein complexes.
• Drug Discovery Screening: Can be utilized in assays to identify inhibitors or modulators of ATP-binding targets.

Basic Information

Product Name	8-(2,2,6,6-Tetramethylpiperidin-4-Yl-1-Oxyl)Amino-Adenosine Triphosphate
CAS No.	80538-64-3
Molecular Formula	C20H33N8O14P3
Molecular Weight	678.45 g/mol
Synonyms	8-((2,2,6,6-Tetramethylpiperidin-1-oxyl-4-yl)amino)adenosine 5'-triphosphate; 8-(4-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl)adenosine triphosphate; SL-ATP; Spin-labeled ATP; TEMPO-ATP; ATP spin label; 8-(TEMPO-amino)-ATP; Adenosine 5'-triphosphate, 8-[(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)amino]-
EINECS	Contact for details

Quality Control

Our 8-(2,2,6,6-Tetramethylpiperidin-4-Yl-1-Oxyl)Amino-Adenosine Triphosphate is produced under strict quality protocols to ensure high purity and batch-to-batch consistency, which is critical for sensitive spectroscopic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, radical concentration, and impurity profiles. We adhere to rigorous analytical standards, utilizing techniques such as HPLC, NMR, and EPR to validate product identity and performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep desiccated at the recommended temperature.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (EPR)	Conforms
Purity (HPLC)	≥ 95%
Radical Concentration	≥ 90%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.