Description

Topoisomerase I CAS NO 80449-01-0 is a critical enzyme that functions by creating transient single-strand breaks in DNA to relieve torsional stress during replication and transcription. This product is essential for research and development in molecular biology, biochemistry, and oncology. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers for applications ranging from drug discovery to fundamental genetic studies.

Application

• Drug Discovery & Screening: Used as a target enzyme in high-throughput assays to identify and characterize novel Topoisomerase I inhibitors for cancer chemotherapy.
• Molecular Biology Research: Essential for studying DNA topology, supercoiling, and the mechanisms of DNA replication and transcription in vitro.
• Biochemical Reagent: Serves as a key component in enzymatic reaction kits for DNA manipulation and analysis.
• Oncology Research: Employed to investigate the mechanism of action of camptothecin and its derivatives, which target Topoisomerase I-DNA complexes.
• Diagnostic Development: Used in the development of assays to measure enzyme activity or inhibitor potency in clinical and research samples.
• Enzyme Characterization Studies: For kinetic analysis, stability profiling, and functional studies of enzyme mutants.

Basic Information

Product Name	Topoisomerase I
CAS No.	80449-01-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DNA Topoisomerase I; Topo I; Relaxing Enzyme; DNA Topoisomerase (ATP-Hydrolysing); EC 5.99.1.2; Type I DNA Topoisomerase; Nicking-Closing Enzyme; Swivelase; Untwisting Enzyme
EINECS	Contact for details

Quality Control

Our Topoisomerase I is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment, and absence of contaminating nuclease activities. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, buffer composition, and storage conditions is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect enzymatic activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Positively relaxes supercoiled DNA
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Contaminating Nuclease	Not detected
Endotoxin	< 1.0 EU/μg (if applicable)
Buffer	50 mM Tris-HCl, pH 7.5, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.