Description

Bis(4-Methylumbelliferyl)Pyrophosphate is a specialized biochemical reagent, a fluorogenic substrate designed for the sensitive detection and quantification of enzymatic activity. Its core value lies in its ability to generate a highly fluorescent product upon enzymatic hydrolysis, enabling precise, real-time kinetic assays. This compound is essential for researchers and quality control laboratories in the life sciences, pharmaceutical development, and diagnostic industries who require reliable tools for studying phosphatases, pyrophosphatases, and related enzymes.

Application

• Enzyme Activity Assays: Primary substrate for continuous fluorometric assays of inorganic pyrophosphatases and acid phosphatases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify enzyme inhibitors or activators for therapeutic target validation.
• Biochemical Research: A critical tool for kinetic studies, enzyme characterization, and mechanistic investigations in molecular biology.
• Diagnostic Development: Serves as a key component in developing fluorogenic probe systems for clinical diagnostics and biosensors.
• Quality Control in Biotechnology: Employed to monitor enzyme purity and activity during biomanufacturing and downstream processing.

Basic Information

Product Name	Bis(4-Methylumbelliferyl)Pyrophosphate
CAS No.	80284-77-1
Molecular Formula	C21H16O11P2
Molecular Weight	506.29 g/mol
Synonyms	4-Methylumbelliferyl Pyrophosphate; Bis(4-MU)PP; Di(4-methylumbelliferyl) pyrophosphate; 4-Methylumbelliferyl Diphosphate; BMUPP; Fluorogenic Pyrophosphate Substrate; 4-MU-PP; PPi-4MU
EINECS	Contact for details

Quality Control

Our Bis(4-Methylumbelliferyl)Pyrophosphate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. For long-term stability, keep the product under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorogenic Activity	Functional in standard pyrophosphatase assay
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.