Description

4-Methylumbelliferyl Thymidine 5'-Phosphate is a specialized fluorogenic substrate used in enzymatic assays. This compound is critical for researchers and diagnostic developers requiring high-sensitivity detection of enzymatic activity. It is primarily utilized in life science research, clinical diagnostics, and pharmaceutical development for applications such as nuclease and phosphatase activity studies.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for detecting and quantifying the activity of nucleases, phosphatases, and other hydrolytic enzymes.
• Biochemical Research: Used in mechanistic studies of enzyme kinetics, inhibition, and substrate specificity in academic and industrial R&D laboratories.
• Diagnostic Kit Development: Employed in the formulation of high-sensitivity diagnostic assays for clinical and research use.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential therapeutic compounds that modulate target enzyme activity.
• Quality Control in Biotechnology: Applied in the quality assessment of enzyme preparations and recombinant proteins.

Basic Information

Product Name	4-Methylumbelliferyl Thymidine 5'-Phosphate
CAS No.	80180-70-7
Molecular Formula	C22H23N2O12P
Molecular Weight	538.40 g/mol
Synonyms	4-MU-TMP; 4-Methylumbelliferyl-5'-thymidylate; Thymidine 5'-phosphate 4-methylumbelliferyl ester; 4-Methylumbelliferyl thymidine monophosphate; MU-TMP; 4-MU-thymidine phosphate; Fluorogenic thymidine phosphate substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Thymidine 5'-Phosphate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee reproducible performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.