Description

4-Methylumbelliferyl Thymidine 3'-Phosphate is a specialized fluorogenic substrate used in enzymatic assays. This compound is critical for the sensitive detection and quantification of nuclease and phosphatase activity. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and molecular diagnostics sectors for high-throughput screening and biochemical research.

Application

• Fluorogenic Substrate for Nucleases: Used to assay the activity of enzymes like S1 nuclease, P1 nuclease, and micrococcal nuclease.
• Phosphatase Activity Assays: Serves as a substrate for alkaline phosphatase and other phosphatases in ELISA and other detection systems.
• High-Throughput Screening (HTS): Enables the screening of compound libraries for inhibitors or activators of specific nucleases or phosphatases in drug discovery.
• Molecular Biology Research: Employed in studies of DNA degradation, repair mechanisms, and nucleotide metabolism.
• Diagnostic Kit Development: A key component in the development of clinical diagnostic assays for various diseases.
• Quality Control in Biomanufacturing: Used to monitor the presence and activity of specific enzymes during biopharmaceutical production processes.

Basic Information

Product Name	4-Methylumbelliferyl Thymidine 3'-Phosphate
CAS No.	80180-69-4
Molecular Formula	C₂₁H₂₃N₂O₁₁P
Molecular Weight	510.39 g/mol
Synonyms	4-MU-TMP; 4-Methylumbelliferyl 5'-Thymidylate; Thymidine 3'-(4-Methylumbelliferyl Phosphate); 4-Methylumbelliferyl Thymidine 3'-Monophosphate; 3'-TMP-4-MU; Fluorogenic Thymidine Phosphate; 4-MU-Thymidine-3'-P; 4-Methylumbelliferyl-3'-thymidylic acid
EINECS	Contact for details

Quality Control

Every batch of 4-Methylumbelliferyl Thymidine 3'-Phosphate is manufactured and tested under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles to ensure it meets the exacting standards required for research and diagnostic applications. Our quality commitment supports compliance with ISO 9001 and relevant Good Manufacturing Practice (GMP) guidelines for critical raw materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional by enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.