Description

5-Bromo-3-Indolyl Phosphate p-Toluidine Salt is a specialized chromogenic substrate widely used in molecular biology and diagnostic applications. Its primary value lies in its enzymatic conversion to an insoluble, intensely colored precipitate, making it an essential reagent for sensitive detection in blotting and histochemical staining techniques. This compound is critically needed by manufacturers of diagnostic kits, research laboratories in life sciences, and academic institutions engaged in protein and nucleic acid analysis.

Application

• Western Blotting: As a key substrate for alkaline phosphatase (AP) conjugated antibodies, producing a stable purple/blue precipitate for protein detection.
• Northern & Southern Blotting: For the colorimetric detection of nucleic acids using AP-labeled probes.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed in colorimetric endpoint detection systems utilizing AP enzymes.
• In Situ Hybridization (ISH): Used in histological sections for the localization of specific DNA or RNA sequences.
• Immunohistochemistry (IHC): As a chromogen for visualizing antigen-antibody complexes in tissue samples.
• Lateral Flow Assays: Incorporated into rapid diagnostic test strips for visual readouts.
• Research & Development: For developing and optimizing new bioanalytical methods and diagnostic platforms.
• Manufacturing of Reagents: As a critical raw material for producing ready-to-use substrate solutions and detection kits.

Basic Information

Product Name	5-Bromo-3-Indolyl Phosphate p-Toluidine Salt
CAS No.	80008-69-1
Molecular Formula	C15H16BrN2O4P
Molecular Weight	399.18 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP p-toluidine salt; 5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; X-Phosphate; BCIP; 5-Br-4-Cl-3-indolyl phosphate p-toluidine salt; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate compound with 4-methylaniline (1:1)
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indolyl Phosphate p-Toluidine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet stringent research and diagnostic-grade specifications. Certificates of Analysis (COA) detailing all test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Performance Test	Suitable for use in blotting applications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.