Description

Diethylumbelliferyl Phosphate is a specialized fluorogenic substrate and chemical intermediate of significant importance in biochemical and diagnostic research. Its core value lies in its enzymatic conversion to a highly fluorescent product, making it an essential tool for sensitive detection and quantification. This compound is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme activity assays, particularly for phosphatases and related hydrolytic enzymes.

Application

• Fluorogenic Substrate for Enzymatic Assays: Primarily used as a sensitive substrate for detecting and quantifying the activity of alkaline phosphatase, acid phosphatase, and other phosphohydrolases in research and diagnostic applications.
• Diagnostic Reagent Manufacturing: A key component in the formulation of ELISA kits, clinical chemistry reagents, and point-of-care diagnostic tests for various biomarkers.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for screening compound libraries against phosphatase targets in drug discovery programs.
• Cell Biology & Signal Transduction Research: Used to study intracellular phosphatase activity and cellular signaling pathways in live or fixed cells.
• Biochemical Research & Method Development: Serves as a standard and model substrate for developing new fluorometric assay protocols and studying enzyme kinetics.
• Quality Control in Biotechnology: Applied in the quality control testing of enzyme preparations and recombinant proteins to assess specific activity and purity.

Basic Information

Product Name	Diethylumbelliferyl Phosphate
CAS No.	897-83-6
Molecular Formula	C13H15O7P
Molecular Weight	314.23 g/mol
Synonyms	4-Methylumbelliferyl Phosphate Diethyl Ester; Diethyl 4-Methylumbelliferyl Phosphate; 4-MUDP; DEP; 7-Hydroxy-4-methylcoumarin Diethyl Phosphate; 4-Methylumbelliferyl Diethyl Phosphate; Umbelliferyl Phosphate Diethyl Ester; Phosphoric Acid, Diethyl Ester with 7-Hydroxy-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Diethylumbelliferyl Phosphate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results for purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Fluorogenic Activity	Functional in standard phosphatase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.