Description

L-Leucine β-Naphthylamide Hydrochloride is a specialized chromogenic substrate used extensively in biochemical research and diagnostic applications. Its core value lies in its role as a specific and sensitive reagent for the detection and quantification of leucine aminopeptidase (LAP) enzyme activity. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors who require reliable, high-purity substrates for enzymatic assays and diagnostic kit formulations.

Application

• Enzymatic Substrate for Leucine Aminopeptidase (LAP): Primary reagent for colorimetric and fluorometric assays to measure LAP activity in biological samples.
• Clinical Diagnostics: Key component in diagnostic kits for liver and pancreatic function tests, where LAP levels serve as a biomarker.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and proteomics research to investigate aminopeptidase function.
• Pharmaceutical Development: Employed in drug discovery pipelines for high-throughput screening of compounds targeting aminopeptidases.
• Microbiology: Applied in microbial identification systems that utilize specific enzymatic profiles.
• Histochemistry: Serves as a substrate for the localization of LAP activity in tissue sections.

Basic Information

Product Name	L-Leucine β-Naphthylamide Hydrochloride
CAS No.	893-36-7
Molecular Formula	C₁₆H₂₀N₂O•HCl
Molecular Weight	284.80 g/mol (Free base: 256.35)
Synonyms	L-Leucyl-2-naphthylamide hydrochloride; L-Leucyl-β-naphthylamide HCl; Leucyl-β-naphthylamide hydrochloride; LAP Substrate; L-Leucine-4-(2-naphthyl)azobenzene hydrochloride; N-(2-Naphthyl)-L-leucinamide hydrochloride; 1-Naphthalenamine, N-(1-oxo-4-methyl-1-[(phenylamino)carbonyl]pentyl)-, hydrochloride (1:1)
EINECS	213-300-6

Quality Control

Our L-Leucine β-Naphthylamide Hydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and verification of enzymatic activity. Certificates of Analysis (COA) detailing all specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
Enzymatic Activity	Conforms to reference standard
Solubility	Soluble in water, DMSO, and methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.