Description

Sphingosine 1-Phosphate Biotin is a high-purity, biotin-conjugated derivative of the bioactive lipid signaling molecule Sphingosine 1-Phosphate (S1P). This compound is specifically engineered for advanced biochemical research and assay development, leveraging the high-affinity interaction of biotin with streptavidin for detection, isolation, and quantification purposes. It is an essential tool for researchers in pharmaceutical discovery, molecular biology, and immunology who are investigating the S1P signaling pathway, its receptors, and related therapeutic targets.

Application

• Biochemical Assay Development: Used as a labeled ligand in ELISA, pull-down assays, and other affinity-based detection systems to study S1P receptor binding and activity.
• Signal Transduction Research: A critical reagent for probing the S1P signaling pathway, including its role in cell proliferation, migration, and immune response regulation.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel agonists or antagonists of S1P receptors.
• Affinity Purification: Facilitates the isolation and purification of S1P-binding proteins or receptor complexes using streptavidin-coated beads or columns.
• Cellular Imaging Studies: Can be utilized in conjunction with fluorescently labeled streptavidin for visualizing S1P receptor localization and trafficking in cells.
• Academic and Industrial R&D: Serves as a key reference standard and research tool in laboratories focused on lipidomics, oncology, and autoimmune disease research.

Basic Information

Product Name	Sphingosine 1-Phosphate Biotin
CAS No.	799812-63-8
Molecular Formula	C38H68N4O9PS
Molecular Weight	796.99 g/mol
Synonyms	Biotin-S1P; Biotinylated Sphingosine 1-Phosphate; S1P-Biotin; N-((6-(Biotinoyl)amino)hexanoyl)-D-erythro-sphingosine-1-phosphate; D-erythro-Sphingosine-1-phosphate, N-[6-[(5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl)amino]hexanoyl]-; Biotinamidocaproyl S1P; S1P conjugated to Biotin
EINECS	Contact for details

Quality Control

Every batch of Sphingosine 1-Phosphate Biotin is manufactured under strict quality protocols and undergoes comprehensive analytical testing to ensure identity, purity, and consistency. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, MS, and NMR analyses. Our quality system is designed to meet the rigorous demands of research and pre-clinical development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be kept under desiccant in the original container. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, ethanol; slightly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.