Description

Chromatide™ Texas Red®-5-dUTP is a fluorescently labeled nucleotide analog designed for high-sensitivity detection in molecular biology applications. This compound is essential for creating non-radioactive, fluorescent probes for techniques like fluorescence in situ hybridization (FISH) and DNA microarray analysis. It is a critical reagent for researchers and diagnostic developers in the life sciences, pharmaceuticals, and clinical diagnostics industries who require precise and reliable nucleic acid labeling.

Application

• Fluorescence In Situ Hybridization (FISH): Primary application for labeling DNA probes to visualize specific genetic sequences on chromosomes or within cells.
• DNA Microarray Fabrication: Used to synthesize fluorescently tagged cDNA or cRNA targets for gene expression profiling and genomic analysis.
• Next-Generation Sequencing (NGS): Can be incorporated for specialized sequencing applications requiring fluorescent detection, such as certain single-molecule sequencing platforms.
• PCR Probe Labeling: Enzymatic incorporation into oligonucleotide probes for real-time PCR (qPCR) and other amplification-based detection assays.
• Cell-Based Assays & Cytogenetics: Enables the direct visualization and quantification of nucleic acids in fixed cells and tissue sections.
• Diagnostic Kit Manufacturing: A key raw material for producing commercial in vitro diagnostic (IVD) tests and research-use-only (RUO) kits.

Basic Information

Product Name	Chromatide™ Texas Red®-5-dUTP
CAS No.	791570-52-0
Molecular Formula	C₃₅H₃₈N₄O₁₇P₃
Molecular Weight	912.61 g/mol
Synonyms	Texas Red®-5-dUTP; Texas Red®-5-2'-deoxyuridine-5'-triphosphate; 5-(Texas Red-X)aminoallyl-2'-deoxyuridine-5'-triphosphate; 5-(Texas Red®-X)-dUTP; TR-5-dUTP; Texas Red®-dUTP; Texas Red®-labeled dUTP; Fluorescent dUTP analog
EINECS	Contact for details

Quality Control

Every batch of Chromatide™ Texas Red®-5-dUTP is manufactured under strict quality systems and undergoes comprehensive analytical testing to ensure performance and consistency. Our products are suitable for research and diagnostic applications, with quality standards aligned with ISO 13485 principles for medical device manufacturing. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, fluorescence properties, and functional performance in enzymatic incorporation assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider storing desiccated under an inert atmosphere.

Specification

Item	Specification
Appearance	Red to dark red solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (fluorophore-labeled nucleotide)
Extinction Coefficient (ε)	~82,000 cm⁻¹M⁻¹ at 595 nm (in aqueous buffer)
Emission Maximum	~615 nm (when bound to DNA)
Enzymatic Incorporation	Suitable for use with Terminal Transferase and DNA Polymerases
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.