Description

Fmoc-L-Lys(Ac)-Amc CAS NO 787549-21-7 is a protected amino acid derivative, specifically an Fmoc-protected lysine residue with an acetylated side chain and an AMC (7-amino-4-methylcoumarin) fluorophore. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled incorporation of a fluorescently labeled lysine into peptide sequences. It is primarily utilized by research scientists and process developers in the pharmaceutical and biotechnology sectors for creating fluorescently tagged peptides used in protease assays, enzyme kinetics studies, and high-throughput screening applications.

Application

• Protease Substrate Synthesis: A key fluorogenic substrate component for synthesizing peptides used to measure the activity of proteolytic enzymes like caspases, trypsin, and cathepsins.
• Enzyme Kinetics & Mechanism Studies: Enables the creation of custom fluorescent peptide substrates to investigate enzyme specificity, inhibition, and catalytic efficiency.
• High-Throughput Screening (HTS) Assays: Used to develop sensitive, homogeneous assays for drug discovery campaigns targeting various proteases.
• Biomarker & Diagnostic Probe Development: Facilitates the construction of peptide-based probes for detecting disease-specific protease activity in clinical and research settings.
• Solid-Phase Peptide Synthesis (SPPS): Serves as a specialized, orthogonally protected amino acid derivative for the Fmoc-strategy, allowing site-specific introduction of a fluorescent label.
• Cell Biology & Imaging: Used to generate cell-permeable peptide substrates for real-time visualization of protease activity within living cells.

Basic Information

Product Name	Fmoc-L-Lys(Ac)-Amc
CAS No.	787549-21-7
Molecular Formula	C₃₃H₃₅N₃O₈
Molecular Weight	601.65 g/mol
Synonyms	Fmoc-Lys(Ac)-AMC; Nα-Fmoc-Nε-acetyl-L-lysine 7-amido-4-methylcoumarin; Fmoc-L-Lysine(Ac)-7-amido-4-methylcoumarin; N-(9-Fluorenylmethoxycarbonyl)-Nε-acetyl-L-lysine 4-methylcoumarin-7-ylamide; Fmoc-Lys(Ac)-AMC Fluorogenic Substrate; Fmoc-Nε-acetyl-L-lysine-AMC; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonylamino)-6-acetamidohexanoic acid 2-oxo-2H-chromen-7-ylamide
EINECS	Contact for details

Quality Control

Our Fmoc-L-Lys(Ac)-Amc is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research or cGMP guidelines upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.