Description

o-[2-(Boc-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 5000 is a heterobifunctional polyethylene glycol (PEG) derivative engineered for advanced bioconjugation and crosslinking applications. This compound is critical for creating stable, site-specific linkages between biomolecules, such as proteins and peptides, while maintaining their biological activity. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-purity, functionalized PEGs for drug delivery systems, protein modification, and surface functionalization.

Application

• Bioconjugation Reagent: For the site-specific crosslinking of proteins, peptides, and antibodies to other molecules or surfaces.
• PEGylation Linker: Used to attach polyethylene glycol chains to therapeutic proteins and peptides to enhance solubility, stability, and circulation half-life.
• Drug Delivery System Development: A key component in constructing advanced drug delivery platforms, including antibody-drug conjugates (ADCs) and nanoparticle formulations.
• Surface Functionalization: For modifying biosensor surfaces, microarray slides, and other solid supports to create bioactive interfaces.
• Diagnostic Assay Development: Employed in the preparation of labeled antibodies and antigens for immunoassays (e.g., ELISA).
• Biomaterial Modification: To introduce functional groups onto hydrogels and other polymeric biomaterials for controlled cell interaction.

Basic Information

Product Name	o-[2-(Boc-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 5000
CAS No.	778648-12-7
Molecular Formula	CnH2n+2On+1 (PEG core) with functional groups
Molecular Weight	~5000 Da (nominal)
Synonyms	Boc-NH-PEG-NHS Ester 5000; Boc-Amino-PEG5000-NHS Ester; Heterobifunctional PEG5000 (Boc-amine, NHS ester); α-(2-Boc-aminoethyl)-ω-(3-(N-succinimidyloxy)-3-oxopropyl)poly(ethylene glycol); PEG5k-Boc-NH2-NHS; Boc-PEG5000-NHS; N-(tert-Butoxycarbonyl)-aminoethyl-PEG5000-N-hydroxysuccinimide ester; Boc-NH-PEG5k-SCM
EINECS	Contact for details

Quality Control

Our o-[2-(Boc-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 5000 is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive research and development applications. Each lot is characterized by advanced analytical techniques including NMR, HPLC, and MALDI-TOF mass spectrometry to confirm functional group integrity, molecular weight distribution, and high purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis of the active NHS ester group. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white waxy solid or powder
Identification (NMR)	Conforms to structure
Nominal Molecular Weight	5000 Da
Molecular Weight Distribution (Đ)	≤ 1.05
Purity (HPLC)	≥ 95%
Functional Group Substitution	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.