Description

(+)-Biotin-Peg4-Hydrazide CAS NO 756525-97-0 is a high-purity, functionalized biotin derivative featuring a tetraethylene glycol (PEG4) spacer and a terminal hydrazide group. This molecular design is critical for creating stable conjugates with aldehydes and ketones, enabling precise biotinylation in aqueous conditions. It is an essential reagent for researchers and manufacturers in bioconjugation, diagnostic assay development, and targeted drug delivery systems, where controlled labeling and enhanced solubility are paramount.

Application

• Bioconjugation Reagent: For site-specific labeling of antibodies, proteins, and peptides via carbonyl groups (aldehydes/ketones) generated by oxidation.
• Diagnostic Kit Development: Used to create biotinylated detection probes for ELISA, lateral flow assays, and other immunoassay platforms.
• Affinity Chromatography Ligand Synthesis: Immobilization onto solid supports for the purification of target biomolecules using streptavidin-biotin interaction.
• Drug Delivery & Targeting: Conjugation to drug molecules or nanoparticles to create biotinylated constructs for avidin-biotin technology-based targeting.
• Surface Functionalization: Modification of sensor chips, microarrays, and other surfaces for biomolecule capture and analysis.
• Chemical Biology Probes: Tool compound for studying protein interactions, localization, and turnover through bioorthogonal labeling strategies.

Basic Information

Item	Detail
Product Name	(+)-Biotin-Peg4-Hydrazide
CAS No.	756525-97-0
Molecular Formula	C23H43N5O7S
Molecular Weight	533.68 g/mol
Synonyms	Biotin-PEG4-hydrazide; Biotinamidohexanoyl-PEG4-hydrazide; (+)-Biotinyl-3,6,9,12-tetraoxapentadecan-15-oic hydrazide; Biotin-PEG4-NHNH2; Biotinylated PEG4 hydrazide; N-(Biotinoyl)-N'-(PEG4-acyl) hydrazine; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-(2-hydrazinyl-2-oxoethoxy)ethoxy]ethoxy]ethoxy]ethyl]pentanamide
EINECS	Contact for details

Quality Control

Our (+)-Biotin-Peg4-Hydrazide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional group analysis. We provide full traceability and Certificates of Analysis (COA) are available for all orders, ensuring compliance with research and cGMP-grade material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.