Description

Nhs-Bib CAS NO 728034-24-0 is a specialized bifunctional crosslinking reagent featuring an N-hydroxysuccinimide (NHS) ester group and a biotin moiety. This compound is critical for creating stable, high-affinity conjugates in bioconjugation workflows, enabling the specific labeling and isolation of target biomolecules. It is an essential tool for researchers and manufacturers in the fields of proteomics, diagnostics, and targeted drug delivery who require reliable and efficient biotinylation strategies.

Application

• Antibody Biotinylation: For labeling primary or secondary antibodies used in ELISA, Western blotting, and immunohistochemistry (IHC) applications.
• Protein and Peptide Labeling: Enables the covalent attachment of biotin to amine-containing proteins, peptides, or other ligands for downstream purification or detection via streptavidin.
• Affinity Probe Development: Used to create biotinylated probes for pull-down assays, mass spectrometry-based interactome studies, and target identification.
• Diagnostic Assay Development: A key reagent in the manufacture of lateral flow assays, biosensors, and other diagnostic kits requiring stable biotin-streptavidin interactions.
• Surface Functionalization: For immobilizing biomolecules onto biosensor chips, microarrays, or nanoparticle surfaces via amine-reactive chemistry.
• Drug Conjugate Research: Facilitates the development of biotin-drug conjugates for exploring avidin-biotin technology in targeted therapeutic delivery systems.

Basic Information

Product Name	Nhs-Bib
CAS No.	728034-24-0
Molecular Formula	C24H30N4O8S
Molecular Weight	534.58 g/mol
Synonyms	Biotin-PEG3-NHS Ester; Biotinamidohexanoyl-6-aminohexanoyl-PEG3-NHS; Biotin-(PEG)3-NHS; Biotin-PEG3-NHS Ester; NHS-PEG3-Biotin; N-Succinimidyl 6-((6-((6-(biotinamido)hexanoyl)amino)hexanoyl)amino)hexanoate; 6-((6-((6-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)amino)hexanoyl)amino)hexanoic acid N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our Nhs-Bib is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to relevant quality management standards to support our global customer base in pharmaceutical research and in vitro diagnostics.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.