Description

15-[D(+)-Biotinylamino]-4,7,10,13-Tetraoxapentadecanoic Acid is a high-purity, synthetic biotin derivative featuring a hydrophilic tetraethylene glycol spacer arm. This compound is engineered to facilitate the biotinylation of biomolecules, offering enhanced solubility and reduced steric hindrance compared to traditional biotinylation reagents. It is an essential tool for researchers and manufacturers in the life sciences sector, particularly for applications in molecular diagnostics, affinity purification, and targeted drug delivery systems.

Application

• Bioconjugation and Labeling: Primary reagent for the site-specific biotinylation of proteins, antibodies, peptides, and nucleic acids for detection and capture assays.
• Affinity Chromatography: Used to functionalize solid supports (e.g., agarose beads) for the purification of streptavidin/avidin-tagged biomolecules.
• Diagnostic Assay Development: A key component in the manufacture of ELISA, lateral flow tests, and other immunoassay platforms requiring stable biotin-streptavidin interactions.
• Drug Delivery Systems: Enables the creation of biotinylated liposomes, nanoparticles, and antibody-drug conjugates (ADCs) for targeted therapeutic approaches.
• Cell Surface Engineering: Facilitates the labeling and isolation of specific cell populations in flow cytometry and cell sorting applications.
• Microarray and Biosensor Fabrication: Used to immobilize probe molecules on sensor surfaces with high specificity and orientation.

Basic Information

Product Name	15-[D(+)-Biotinylamino]-4,7,10,13-Tetraoxapentadecanoic Acid
CAS No.	721431-18-1
Molecular Formula	C24H43N3O8S
Molecular Weight	533.68 g/mol
Synonyms	Biotin-PEG4-acid; Biotin-amido-PEG4-acid; Biotin-PEG4-COOH; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(17-carboxy-3,6,9,12-tetraoxahexadec-1-yl)pentanamide; Biotinylated Tetraethylene Glycol Acid; EZ-Link Biotin-PEG4-acid; PEG4-Biotin
EINECS	Contact for details

Quality Control

Our 15-[D(+)-Biotinylamino]-4,7,10,13-Tetraoxapentadecanoic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency, meeting the high standards required for research and in-vitro diagnostic use. A comprehensive Certificate of Analysis (COA) detailing HPLC purity, NMR confirmation, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Under these conditions, the product is stable for at least 24 months. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.