Description

5-Carboxyfluorescein Diacetate is a cell-permeable, non-fluorescent ester derivative of fluorescein, widely used as a vital intracellular probe. Upon entering living cells, endogenous esterases cleave the acetate groups, releasing the highly fluorescent 5-carboxyfluorescein, enabling real-time monitoring of cell viability, membrane integrity, and cytoplasmic tracking. This compound is essential for researchers and manufacturers in cell biology, high-throughput screening, and diagnostic assay development, providing a reliable tool for quantifying enzymatic activity and cellular health.

Application

• Cell Viability and Cytotoxicity Assays: As a standard substrate for intracellular esterase activity, indicating live cells.
• Cell Tracing and Migration Studies: For long-term tracking of cell populations in vitro and in vivo.
• Membrane Permeability and Integrity Tests: Used to assess pore formation, membrane damage, and dye leakage.
• Flow Cytometry and Fluorescence Microscopy: A key fluorescent indicator for quantitative analysis of cell function.
• High-Content Screening (HCS): Employed in automated platforms for drug discovery and toxicology studies.
• Enzyme Kinetics: Studying esterase enzyme activity and inhibition in biochemical research.
• Diagnostic Kit Manufacturing: A critical component in kits for clinical and research laboratory use.
• Neuroscience Research: For labeling neurons and studying neuronal connectivity and function.

Basic Information

Product Name	5-Carboxyfluorescein Diacetate
CAS No.	79955-27-4
Molecular Formula	C25H16O9
Molecular Weight	460.39 g/mol
Synonyms	5(6)-Carboxyfluorescein Diacetate; CFDA; 5-CFDA; Carboxyfluorescein Diacetate; 3',6'-Diacetyl-5(6)-carboxyfluorescein; 5(6)-Carboxy-2',7'-dichlorofluorescein Diacetate (common isomer mixture); Fluorescein Diacetate 5(6)-Carboxy; CellTrace™ CFDA SE (related tracking dye)
EINECS	Contact for details

Quality Control

Our 5-Carboxyfluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biological applications. A comprehensive Certificate of Analysis (COA) detailing purity, performance in functional assays, and absence of critical impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Under these conditions, the product is stable for extended periods. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (typical ≥ 98%)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Functional Performance	Conforms in standard cell-loading assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.