Description

n-Succinimidyl 4-(4-Maleimidophenyl)Butyrate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This dual functionality is critical for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay development, where precise and controlled linkage is required.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing stable, cleavable, or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: Enables the covalent coupling of different proteins or peptides, useful for creating bispecific constructs or protein-polymer hybrids.
• Enzyme and Protein Immobilization: Facilitates the site-directed attachment of enzymes or other proteins to solid supports (e.g., beads, plates, sensors) for diagnostics and biocatalysis.
• Fluorescent/Detection Probe Labeling: Used to attach fluorophores, biotin, or other reporter molecules to antibodies, proteins, or oligonucleotides for imaging and detection applications.
• Polymer and Biomaterial Functionalization: Serves as a crosslinker to introduce reactive maleimide groups into polymers or hydrogels for subsequent bioconjugation.
• Diagnostic Reagent Development: Critical in the synthesis of components for immunoassays (ELISA, lateral flow) and other diagnostic platforms.

Basic Information

Product Name	n-Succinimidyl 4-(4-Maleimidophenyl)Butyrate
CAS No.	79886-55-8
Molecular Formula	C18H16N2O6
Molecular Weight	356.33 g/mol
Synonyms	4-(N-Maleimidophenyl)butyric acid N-succinimidyl ester; SMPB; Succinimidyl 4-(p-maleimidophenyl)butyrate; N-Succinimidyl 4-maleimidobutyrate (SMB) derivative; 4-Maleimidobutyric acid N-hydroxysuccinimide ester; Maleimidobenzoyl-N-hydroxysuccinimide ester; SM(PEG)n crosslinker precursor; NHS-PEG4-Maleimide analog; Heterobifunctional crosslinker, NHS-Maleimide type
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 4-(4-Maleimidophenyl)Butyrate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our customers in regulated development pathways.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the maleimide group can degrade upon prolonged exposure to moisture or elevated temperatures. For long-term storage, keep containers sealed under an inert atmosphere (e.g., argon or nitrogen). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.