Description

4-Nitrophenyl 2-o-α-D-Galactopyranosyl-α-D-Mannopyranoside is a specialized synthetic glycoside derivative, serving as a critical chromogenic substrate for enzymatic assays. Its core value lies in enabling precise, colorimetric detection and quantification of specific glycosidase enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, where accurate enzyme characterization is paramount.

Application

• Enzymatic Substrate for Glycosidases: Primary use as a chromogenic substrate for α-galactosidase and related enzymes in biochemical research.
• Diagnostic Kit Development: Key component in the formulation of diagnostic assays and kits for detecting enzyme deficiencies or microbial activity.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify potential inhibitors or activators of target glycosidases.
• Biochemical Research & Enzyme Kinetics: Fundamental tool for studying enzyme mechanisms, specificity, and kinetic parameters in vitro.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme preparations used in therapeutic production.
• Microbiology Studies: Applied in assays to characterize glycosidase activity of various bacterial or fungal strains.

Basic Information

Item	Detail
Product Name	4-Nitrophenyl 2-o-α-D-Galactopyranosyl-α-D-Mannopyranoside
CAS No.	79580-50-0
Molecular Formula	C19H25NO13
Molecular Weight	475.40 g/mol
Synonyms	p-Nitrophenyl 2-O-α-D-galactopyranosyl-α-D-mannopyranoside; 4-Nitrophenyl 2-O-α-D-galactosyl-α-D-mannoside; PNPGalαMan; 2-O-α-D-Galactopyranosyl-α-D-mannopyranose 4-nitrophenyl glycoside; 4-Nitrophenyl α-D-galactosyl-(1→2)-α-D-mannoside; Galactosylmannoside 4-nitrophenyl derivative; Chromogenic substrate for α-galactosidase
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 2-o-α-D-Galactopyranosyl-α-D-Mannopyranoside is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and specific optical rotation measurement, to ensure it meets the exacting standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.