Description

5-Propenyl-Dutp is a modified deoxyuridine triphosphate nucleotide analog, a critical building block for advanced biochemical research and diagnostics. This compound is essential for applications requiring the specific incorporation of a propenyl-modified base, enabling precise molecular labeling and probe development. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, next-generation sequencing (NGS), and diagnostic assay development.

Application

• Next-Generation Sequencing (NGS): Used as a modified nucleotide for sequencing-by-synthesis platforms to study nucleic acid interactions or develop novel sequencing chemistries.
• Probe Synthesis: Incorporation into DNA probes for fluorescence in situ hybridization (FISH) or microarray applications to enhance detection specificity.
• Enzymatic Studies: Serves as a substrate for DNA polymerases and reverse transcriptases to investigate enzyme kinetics, fidelity, and inhibition.
• Diagnostic Assay Development: Key component in the creation of sensitive molecular diagnostic tests, including PCR-based assays for pathogen detection.
• Bioconjugation & Labeling: The propenyl group provides a reactive handle for further chemical modification and attachment of reporter molecules like fluorophores or biotin.
• Antiviral Research: Investigated as a potential nucleotide analog for studying viral replication mechanisms and developing therapeutic agents.

Basic Information

Item	Detail
Product Name	5-Propenyl-Dutp
CAS No.	79551-91-0
Molecular Formula	C14H21N2O14P3
Molecular Weight	534.24 g/mol
Synonyms	5-(1-Propenyl)-2'-deoxyuridine-5'-triphosphate; 5-Propenyl-2'-deoxyuridine triphosphate; 5-Propenyl-dUTP; dUTP-5-Propenyl; Modified dUTP; 5-(Prop-1-en-1-yl)-2'-deoxyuridine 5'-(trihydrogen diphosphate); Propenyl-modified nucleotide
EINECS	Contact for details

Quality Control

Every batch of 5-Propenyl-Dutp is manufactured and tested under stringent quality management systems to ensure the highest standards of purity and consistency for research use. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (Nucleotide content)
Water Content (KF)	≤ 5%
Solubility	Soluble in water or TE buffer
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.