Description

22-Nbd Cholesterol is a fluorescently labeled derivative of cholesterol, widely used as a vital molecular probe in biochemical and cell biology research. Its core value lies in enabling the direct visualization and tracking of cholesterol distribution, trafficking, and metabolism within living cells and model membranes. This compound is essential for researchers and professionals in the pharmaceutical, biotechnology, and life science sectors who are investigating lipid dynamics, membrane biophysics, and diseases related to cholesterol homeostasis.

Application

• Fluorescent Labeling & Imaging: Used as a fluorescent tracer to visualize cholesterol localization and movement in live and fixed cells using fluorescence microscopy.
• Membrane Biophysics Studies: Employed to study lipid raft formation, membrane fluidity, and the interaction of cholesterol with other lipids and membrane proteins.
• Intracellular Trafficking Research: Tracks the endocytic and secretory pathways of cholesterol, providing insights into cellular lipid transport mechanisms.
• Drug Discovery & Screening: Serves as a tool in high-content screening assays to evaluate compounds that affect cholesterol synthesis, uptake, or efflux.
• Atherosclerosis & Metabolic Disease Models: Facilitates research into the cellular accumulation of cholesterol, a key factor in cardiovascular and metabolic diseases.
• Model Membrane Systems: Incorporated into liposomes and supported lipid bilayers to create biomimetic systems for controlled experimental studies.

Basic Information

Product Name	22-Nbd Cholesterol
CAS No.	78949-95-8
Molecular Formula	C36H52N4O2
Molecular Weight	572.84 g/mol
Synonyms	22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl))-5-cholesten-3β-ol; NBD-Cholesterol; 22-NBD-Cholestrol; Cholesterol NBD; 22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl))amino-23,24-bisnor-5-cholen-3β-ol; Fluorescent Cholesterol Derivative; 22-NBD-Chol
EINECS	Contact for details

Quality Control

Our 22-Nbd Cholesterol is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for research-grade biochemicals, ensuring reliable performance in your experimental systems.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. Allow the product to equilibrate to room temperature in a desiccator before opening to prevent condensation. Keep the container tightly sealed and away from moisture when not in use.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorescence Properties	Excitation ~465 nm, Emission ~535 nm
Solubility	Soluble in DMSO, DMF, ethanol, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.