Description

Fpg Protein CAS NO 78783-53-6 is a highly purified formamidopyrimidine-DNA glycosylase, a critical enzyme involved in the base excision repair (BER) pathway. This enzyme is essential for the accurate detection and removal of oxidized purines, such as 8-oxoguanine, which are major contributors to mutagenesis and cellular damage. It is a vital reagent for researchers and quality control laboratories in the life sciences, pharmaceutical development, and molecular diagnostics sectors, where DNA integrity and repair mechanisms are studied.

Application

• DNA Repair Mechanism Studies: Fundamental research into the Base Excision Repair (BER) pathway and oxidative DNA damage.
• Biomarker Detection Assays: Used in enzymatic assays to detect and quantify oxidative DNA lesions, serving as biomarkers for oxidative stress.
• Pharmaceutical R&D: Screening and validation of novel compounds that modulate DNA repair activity for potential therapeutic applications.
• Genotoxicity Testing: A key component in in vitro assays (e.g., comet assay modifications) to assess the DNA-damaging potential of chemicals and environmental agents.
• Molecular Diagnostic Kits: Incorporated into diagnostic kits designed to evaluate DNA repair capacity, which can be linked to disease risk and progression.
• Enzyme Activity Standardization: Serves as a reference standard for calibrating activity measurements in research and commercial testing laboratories.

Basic Information

Product Name	Fpg Protein
CAS No.	78783-53-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Formamidopyrimidine-DNA Glycosylase; Fapy-DNA Glycosylase; 8-Oxoguanine DNA Glycosylase; MutM Protein; EC 3.2.2.23; DNA-(apurinic or apyrimidinic site) lyase; Oxoguanine Glycosylase
EINECS	Contact for details

Quality Control

Our Fpg Protein is produced under stringent quality management protocols to ensure batch-to-batch consistency and high enzymatic activity. Each lot is subjected to rigorous purity and functional activity testing. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and absence of contaminating nucleases is provided with every shipment to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot as recommended upon receipt to maintain optimal activity.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 95%
Specific Activity	≥ 50,000 units/mg protein
Protein Concentration	Provided on COA
Buffer Composition	50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5
Endonuclease Activity	Not detected
Exonuclease Activity	Not detected
Functional Test	Cleaves oligonucleotide containing 8-oxoG lesion

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.