Description

Calcifediol-D6 is a deuterated analog of calcifediol, a key intermediate in the vitamin D metabolic pathway. This high-purity, stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring analytical accuracy and reliability. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and diagnostic manufacturers for applications in drug metabolism studies, clinical assay development, and biomedical research.

Application

• Internal Standard for LC-MS/MS: Critical for the precise quantification of endogenous calcifediol levels in clinical and research bioanalytical methods.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in pharmaceutical development to study the absorption, distribution, metabolism, and excretion of vitamin D analogs and related therapeutics.
• Clinical Diagnostics: Enables accurate measurement of 25-hydroxyvitamin D status in serum or plasma for patient health assessment.
• Biomedical Research: Facilitates tracer studies to elucidate the complex metabolic pathways and biological functions of vitamin D.
• Method Development and Validation: Serves as a reference material for developing, calibrating, and validating new analytical protocols in compliance with regulatory guidelines.
• Quality Control in Manufacturing: Supports in-process testing and final product release for companies producing vitamin D metabolites or related pharmaceutical products.

Basic Information

Product Name	Calcifediol-D6
CAS No.	78782-98-6
Molecular Formula	C27H38D6O3
Molecular Weight	422.68 g/mol
Synonyms	25-Hydroxyvitamin D3-d6; 25-Hydroxycholecalciferol-d6; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol-d6; 25(OH)D3-d6; 25-Hydroxyergocalciferol-d6 (common name for analog); 25-Hydroxy Vitamin D3 Deuterated; 25-Hydroxycolecalciferol-d6; 25-OH-Vitamin D3-d6
EINECS	Contact for details

Quality Control

Our Calcifediol-D6 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing purity, isotopic enrichment, and analytical results. We support compliance with GMP/GLP principles for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, keep desiccated to maintain stability. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.