Description

6-Phosphofructo-2-Kinase (CAS No. 78689-77-7) is a critical regulatory enzyme in the glycolytic pathway, specifically catalyzing the synthesis of fructose-2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1. This compound is essential for researchers and developers studying cellular metabolism, energy regulation, and metabolic disorders. It is primarily utilized in biochemical research, pharmaceutical development for metabolic disease targets, and as a key reagent in diagnostic assay development.

Application

• Biochemical Research: Fundamental studies on glycolysis, gluconeogenesis, and the regulation of carbohydrate metabolism.
• Pharmaceutical R&D: Target identification and validation for developing therapeutics against diabetes, cancer, and other metabolic syndromes.
• Diagnostic Development: Used as a critical component in enzymatic assays to measure metabolic pathway activity in clinical and research settings.
• Enzyme Kinetics Studies: Investigation of allosteric regulation, inhibitor screening, and mechanistic studies of phosphofructokinase activity.
• Academic Research: A key reagent for university and institutional labs focusing on cell biology, biochemistry, and molecular physiology.
• Biotech Production: Serves as a reference standard or active ingredient in the production of specialized research kits and biochemicals.

Basic Information

Product Name	6-Phosphofructo-2-Kinase
CAS No.	78689-77-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PFK-2; 6-Phosphofructo-2-kinase; Fructose-2,6-bisphosphatase; PFKFB; 6PF-2-K; Phosphofructokinase 2; TIGAR (TP53-induced glycolysis and apoptosis regulator) related enzyme; EC 2.7.1.105 / EC 3.1.3.46 (bifunctional enzyme)
EINECS	Contact for details

Quality Control

Our 6-Phosphofructo-2-Kinase is produced and handled under stringent quality management protocols to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous analytical testing, including activity assays and purity verification. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and stability data is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	> 50 units/mg protein
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	Contact for details
Endotoxin Level	< 1.0 EU/µg
Solubility	Soluble in appropriate assay buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.