Description

Urokinase Substrate I, Colorimetric is a specialized chromogenic peptide substrate designed for the selective detection and quantification of urokinase-type plasminogen activator (uPA) activity. This compound is critical for biochemical research and diagnostic assay development, providing a reliable and sensitive means to measure uPA enzyme kinetics. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and clinical diagnostics sectors, particularly those focused on oncology, fibrinolysis, and cell migration studies.

Application

• Enzymatic Activity Assays: Primary substrate for the colorimetric determination of urokinase-type plasminogen activator (uPA) activity in research and diagnostic kits.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential uPA inhibitors for cancer therapy.
• Biochemical Research: Fundamental tool for studying the role of the uPA system in physiological and pathological processes such as tumor invasion, metastasis, and tissue remodeling.
• Diagnostic Development: Key component in the development of in vitro diagnostic (IVD) assays for measuring uPA levels in biological samples.
• Quality Control: Employed in pharmaceutical QC labs to test the potency and consistency of urokinase-based therapeutic products.
• Academic Research: Utilized in university and institutional labs for fundamental enzymology and cell signaling pathway studies.

Basic Information

Product Name	Urokinase Substrate I, Colorimetric
CAS No.	78333-16-1
Molecular Formula	C34H47N7O9
Molecular Weight	697.79 g/mol
Synonyms	PyroGlu-Gly-Arg-p-nitroanilide acetate salt; S-2444; L-Pyroglutamyl-glycyl-L-arginine 4-nitroanilide acetate; Chromogenic Substrate S-2444; uPA Substrate; Urokinase Chromogenic Substrate; Benzyloxycarbonyl-Gly-Gly-Arg-pNA (related); H-D-Ile-Pro-Arg-pNA (comparative)
EINECS	Contact for details

Quality Control

Our Urokinase Substrate I, Colorimetric is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive enzymatic assays. Certificates of Analysis (COA) with detailed specifications are provided with every shipment to guarantee traceability, consistency, and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Specific Activity	Contact for details
Extinction Coefficient	~10,000 M-1cm-1 at 405 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.