Description

Mono-Methyl Polyethylene Glycol 5'000 Succinate n-Succinimidyl Ester is a heterobifunctional PEG derivative, featuring a reactive NHS ester group for amine coupling and a methoxy terminus for non-reactive conjugation or as a spacer. This compound is critical for creating stable, biocompatible linkages in bioconjugation chemistry, enhancing the solubility and pharmacokinetic profiles of therapeutic molecules. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for developing advanced drug delivery systems, protein and peptide modification, and diagnostic reagent preparation.

Application

• Bioconjugation Reagent: For the site-specific modification of proteins, peptides, and antibodies via primary amine groups.
• PEGylation Agent: To improve the solubility, stability, and circulation half-life of therapeutic peptides and small molecule drugs.
• Crosslinker for Biomaterials: In the synthesis of hydrogels, functionalized surfaces, and drug-loaded nanoparticles for controlled release applications.
• Diagnostic Probe Synthesis: Conjugation to fluorescent dyes, enzymes, or other reporter molecules to create sensitive assay components.
• Linker for ADC Development: As a component in the construction of Antibody-Drug Conjugates (ADCs) to connect cytotoxic payloads to targeting antibodies.
• Polymer-Protein Conjugate Research: For academic and industrial R&D focused on understanding and optimizing polymer-biomolecule interactions.

Basic Information

Product Name	Mono-Methyl Polyethylene Glycol 5'000 Succinate n-Succinimidyl Ester
CAS No.	78274-32-5
Molecular Formula	CnH2n+1O(C2H4O)mC4H2O3N (Variable, based on PEG chain length)
Molecular Weight	~5,000 Da (Average Mn)
Synonyms	mPEG-SCM; mPEG-Succinimidyl Carboxymethyl Ester; mPEG-Succinimidyl Succinate; mPEG-SC; Methoxy PEG NHS Ester; NHS-PEG-OCH3; Poly(ethylene glycol) methyl ether succinimidyl succinate; PEG-NHS Ester; SC-PEG-OCH3; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]propanoate (for short chain analog)
EINECS	Contact for details

Quality Control

Our Mono-Methyl Polyethylene Glycol 5'000 Succinate n-Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent molecular weight, and functional group integrity, suitable for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications for identity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and the active NHS ester group is susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white waxy solid or powder
Identification (IR)	Conforms to structure
Average Molecular Weight (Mn)	5,000 ± 10% (by GPC/MALDI-TOF)
Purity (HPLC)	≥ 95%
Functional Substitution (NHS Ester)	≥ 90%
Moisture (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.