Description

p-Hydroxyhippuryl-His-Leu-Oh is a synthetic tripeptide derivative, specifically designed as a chromogenic substrate for the study of proteolytic enzymes. This compound matters for its high specificity and sensitivity in enzymatic assays, enabling precise biochemical research and diagnostic development. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzyme kinetics and inhibitor screening.

Application

• Enzymatic Substrate: Used as a specific chromogenic substrate for angiotensin-converting enzyme (ACE) and other related proteases in biochemical assays.
• Pharmaceutical Research: Critical tool in the screening and development of ACE inhibitors and other cardiovascular drugs.
• Diagnostic Development: Employed in the formulation of in vitro diagnostic (IVD) kits for clinical measurement of ACE activity in serum.
• Biochemical Analysis: Facilitates kinetic studies and mechanistic investigations of enzyme function in academic and industrial research settings.
• Quality Control: Serves as a reference standard in the quality control of biopharmaceutical processes involving proteolytic enzymes.

Basic Information

Product Name	p-Hydroxyhippuryl-His-Leu-Oh
CAS No.	77697-23-5
Molecular Formula	C23H28N6O7
Molecular Weight	500.51 g/mol
Synonyms	HHL; Hippuryl-His-Leu-OH; N-Hippuryl-L-Histidyl-L-Leucine; N-Benzoylglycyl-L-histidyl-L-leucine; ACE Substrate; Angiotensin-Converting Enzyme Substrate; p-Hydroxyhippuryl-L-histidyl-L-leucine; Chromogenic ACE Substrate
EINECS	Contact for details

Quality Control

Our p-Hydroxyhippuryl-His-Leu-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your regulatory and quality assurance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Assay	95.0% - 105.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.