Description

p-Nitrophenyl 2-o-(B-L-Fucopyranosyl)-B-D-Galactopyranoside is a specialized chromogenic substrate designed for the sensitive detection and kinetic analysis of specific glycosidase enzymes. This compound matters because it provides a reliable, colorimetric readout for enzymatic activity, which is critical for biochemical research and diagnostic assay development. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical, and diagnostic industries for applications such as enzyme characterization, high-throughput screening, and the study of carbohydrate metabolism.

Application

• Glycosidase Enzyme Assays: Acts as a specific chromogenic substrate for α-L-fucosidase and related enzymes, producing a yellow p-nitrophenol product upon hydrolysis for easy spectrophotometric detection.
• Biochemical Research: Used in kinetic studies to determine enzyme activity, inhibition constants (Ki), and substrate specificity in purified enzyme preparations.
• Diagnostic Kit Development: Employed in the formulation of diagnostic kits for the detection of enzyme deficiencies, such as fucosidosis, in clinical and research laboratories.
• High-Throughput Screening (HTS): Utilized in drug discovery campaigns to identify potential inhibitors of glycosidases, which are targets for various therapeutic areas.
• Quality Control in Enzyme Production: Serves as a standard substrate for the quality assurance and potency testing of commercially produced glycosidase enzymes.
• Carbohydrate Metabolism Studies: Aids in investigating the pathways and mechanisms of complex carbohydrate breakdown in biological systems.

Basic Information

Product Name	p-Nitrophenyl 2-o-(B-L-Fucopyranosyl)-B-D-Galactopyranoside
CAS No.	77640-21-2
Molecular Formula	C18H25NO12
Molecular Weight	447.39 g/mol
Synonyms	4-Nitrophenyl 2-O-(β-L-fucopyranosyl)-β-D-galactopyranoside; pNP-2-O-Fuc-β-Gal; 2'-Fucosyllactose 4-nitrophenyl glycoside; 4-Nitrophenyl 2-O-(6-deoxy-β-L-galactopyranosyl)-β-D-galactopyranoside; p-Nitrophenyl 2-O-(β-L-fucopyranosyl)-β-D-galactopyranoside; Fucosidase substrate; α-L-Fucosidase chromogenic substrate; 77640-21-2
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 2-o-(B-L-Fucopyranosyl)-B-D-Galactopyranoside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.