Description

4-Methylumbelliferyl-B-D-Cellobio CAS NO 77626-61-0 is a high-purity fluorogenic substrate specifically designed for the sensitive detection and quantification of cellulase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise, reliable, and reproducible enzymatic assays. It is primarily utilized in the biotechnology, biofuel research, and pulp & paper industries for studying cellulose degradation and optimizing enzymatic processes.

Application

• Cellulase Enzyme Assays: As a specific fluorogenic substrate for the quantitative measurement of endo- and exo-cellulase (cellobiohydrolase) activity in research and industrial samples.
• Biofuel Research & Development: For screening and characterizing cellulolytic enzymes used in the saccharification of lignocellulosic biomass for bioethanol production.
• Pulp & Paper Industry: To monitor and optimize enzymatic treatments in pulp processing and paper recycling operations.
• Microbiology & Mycology: Used in studies of cellulase-producing microorganisms, including fungi and bacteria, for strain identification and characterization.
• Agricultural Research: For analyzing cellulase activity in soil samples, compost, and studies related to plant biomass decomposition.
• Diagnostic Kit Manufacturing: As a key reagent component in diagnostic kits designed to detect specific enzymatic activities in clinical or environmental settings.
• Food & Feed Industry: For enzyme activity assays relevant to the processing of plant-based materials and feed additives.
• High-Throughput Screening (HTS): Suitable for automated, fluorometric HTS platforms in pharmaceutical and biotechnology discovery pipelines targeting cellulase inhibitors or activators.

Basic Information

Product Name	4-Methylumbelliferyl-B-D-Cellobio
CAS No.	77626-61-0
Molecular Formula	C23H28O14
Molecular Weight	528.46 g/mol
Synonyms	4-Methylumbelliferyl β-D-cellobioside; 4-MU-β-D-cellobioside; 4-Methylumbelliferyl cellobioside; MUC; 4-MU-Cellobioside; 7-Hydroxy-4-methylcoumarin β-D-cellobioside; 4-Methylumbelliferyl-β-cellobioside; Cellobioside, 4-methylumbelliferyl
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-B-D-Cellobio is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive enzymatic assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant industry standards for biochemical reagents to support your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may affect its stability and performance. Keep the container tightly sealed after each use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.