Description

Gdpfucose Synthetase CAS NO 77536-21-1 is a critical enzymatic reagent used in the biosynthesis of GDP-L-fucose, an essential nucleotide sugar donor for fucosylation reactions. This compound is vital for researchers and manufacturers developing glycoconjugates, glycoproteins, and other bioactive molecules where precise fucose incorporation is required. It is primarily utilized by biotechnology firms, pharmaceutical R&D departments, and academic institutions focused on glycoscience, glycoengineering, and the production of therapeutic biologics.

Application

• Glycoprotein and Glycoconjugate Synthesis: Essential for the in vitro or cell-free enzymatic synthesis of fucosylated glycoproteins, antibodies, and other therapeutic biologics.
• Enzymatic Glycoengineering: Used as a key substrate in enzymatic cascades to modify cell surfaces or produce defined glycan structures for research and diagnostic applications.
• Biochemical Research: Serves as a fundamental tool in studying fucosyltransferase activity, glycan biosynthesis pathways, and the role of fucosylation in cell signaling and disease.
• Pharmaceutical Development: Enables the production of fucose-containing drug candidates, including vaccines, enzyme replacement therapies, and targeted cancer treatments.
• Diagnostic Reagent Production: Used in manufacturing kits and reagents for detecting specific glycan biomarkers associated with inflammation, cancer, and other conditions.

Basic Information

Product Name	Gdpfucose Synthetase
CAS No.	77536-21-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	GDP-Fucose Synthetase; GDP-L-Fucose Synthetase; Guanosine Diphosphate Fucose Synthetase; GFS; FX Protein; GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase; EC 1.1.1.271
EINECS	Contact for details

Quality Control

Our Gdpfucose Synthetase is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and absence of contaminating nucleases or proteases. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as protein concentration, enzymatic activity (U/mg), purity by SDS-PAGE, and buffer composition. We adhere to relevant guidelines for research-grade enzymes to ensure batch-to-batch consistency and reliable performance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. Store in a dedicated freezer away from volatile chemicals.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Protein Concentration	≥ 1.0 mg/mL
Specific Activity	≥ 50 U/mg
Purity (SDS-PAGE)	≥ 90%
Buffer	Tris-HCl, Glycerol, DTT
pH	7.5 ± 0.5
Endotoxin Level	< 1.0 EU/μg
Biological Activity	Functional in standard GDP-fucose synthesis assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.