Description

2',3' o-(2,4,6-Trinitrophenyl)Adenosine 5'-Diphosphate is a specialized, high-purity biochemical reagent, a modified nucleotide derivative designed for advanced research applications. This compound is critical for scientists investigating nucleotide-protein interactions, enzyme kinetics, and signal transduction pathways due to its unique trinitrophenyl (TNP) fluorescent labeling group. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on molecular biology, enzymology, and assay development.

Application

• Enzyme Inhibition Studies: Acts as a potent, fluorescently-labeled competitive inhibitor for studying ATP-binding enzymes, including kinases, ATPases, and synthetases.
• Biochemical Assay Development: Serves as a key substrate or inhibitor in high-throughput screening (HTS) assays and fluorescence polarization (FP) assays for drug discovery.
• Protein Binding & Interaction Analysis: Used in fluorescence-based techniques to quantify binding affinities, stoichiometry, and conformational changes in nucleotide-binding proteins.
• Metabolic Pathway Research: A tool compound for probing adenosine diphosphate (ADP)-dependent metabolic processes and energy transfer mechanisms within cells.
• Diagnostic Reagent Component: Employed in the formulation of specialized diagnostic tests that require high-sensitivity detection of enzymatic activity.

Basic Information

Product Name	2',3' o-(2,4,6-Trinitrophenyl)Adenosine 5'-Diphosphate
CAS No.	77450-67-0
Molecular Formula	C16H17N8O17P2
Molecular Weight	646.30 g/mol
Synonyms	TNP-ADP; 2',3'-O-(2,4,6-Trinitrophenyl)adenosine-5'-diphosphate; Trinitrophenyl-ADP; Adenosine-5'-diphosphate, 2',3'-O-(2,4,6-trinitrophenyl)-; TNP-Adenosine-5'-diphosphate; Fluorescent ADP analog; 2',3'-O-(TNP)-ADP; 2',3'-O-(2,4,6-Trinitrophenyl)-ADP
EINECS	Contact for details

Quality Control

Every batch of 2',3' o-(2,4,6-Trinitrophenyl)Adenosine 5'-Diphosphate is manufactured and analyzed under strict quality management systems to ensure consistency and reliability for sensitive research applications. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure compliance with exacting research-grade standards. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (UV/VIS)	Conforms (λmax ~ 408 nm)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.