Description

n-T-Boc-o-Benzyl-Ser-Gly-Arg p-Nitroanilide is a synthetic, chromogenic peptide substrate designed for enzymatic activity studies. This compound is of critical importance in biochemical research and pharmaceutical development as a highly specific tool for the detection and quantification of serine protease activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease inhibitor screening, enzyme kinetics, and assay development.

Application

• Biochemical Research: As a specific substrate for studying the kinetics and inhibition profiles of serine proteases, including thrombin, trypsin, and related enzymes.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitor drug candidates.
• Diagnostic Development: Employed in the formulation of chromogenic assay kits for clinical diagnostics, particularly in coagulation and fibrinolysis testing.
• Enzyme Characterization: Essential for determining the specific activity, purity, and functionality of purified protease preparations in academic and industrial labs.
• Quality Control: Serves as a reference standard in the quality control of therapeutic enzymes and related biopharmaceutical products.

Basic Information

Product Name	n-T-Boc-o-Benzyl-Ser-Gly-Arg p-Nitroanilide
CAS No.	77220-80-5
Molecular Formula	C30H42N8O8
Molecular Weight	642.71 g/mol
Synonyms	Boc-Ser(Bzl)-Gly-Arg-pNA; t-Boc-Ser(Bzl)-Gly-Arg-p-Nitroanilide; N-α-t-Butoxycarbonyl-O-benzyl-L-seryl-glycyl-L-arginine 4-nitroanilide; Chromogenic Substrate for Serine Proteases; Thrombin Substrate Analog; Boc-Ser(Bzl)-Gly-Arg-pNA; Ser-Gly-Arg pNA derivative; Protease Substrate 77220-80-5
EINECS	Contact for details

Quality Control

Our n-T-Boc-o-Benzyl-Ser-Gly-Arg p-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to relevant quality guidelines to support our global clientele in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Optical Rotation	Contact for details
Assay (Enzymatic)	Fully active as a substrate for target proteases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.