Description

4-Hydroxy Triazolam B-D-Glucuronide is a key glucuronide metabolite of the benzodiazepine triazolam. This compound is of significant importance in analytical and forensic chemistry for the development of precise reference standards and calibration materials. It is primarily needed by pharmaceutical research laboratories, forensic toxicology units, and contract research organizations (CROs) engaged in drug metabolism and pharmacokinetic (DMPK) studies.

Application

• Reference Standard for Analytical Testing: Serves as a certified primary standard for the quantitative and qualitative analysis of triazolam and its metabolites in biological matrices.
• Forensic Toxicology: Critical for the accurate identification and confirmation of triazolam use in medico-legal investigations, workplace testing, and post-mortem analyses.
• Drug Metabolism & Pharmacokinetics (DMPK): Used in in vitro and in vivo studies to elucidate the metabolic pathways, excretion profiles, and bioavailability of triazolam.
• Clinical Research: Supports studies on drug-drug interactions, metabolic enzyme activity (e.g., UGT), and the development of therapeutic drug monitoring (TDM) protocols.
• Method Development & Validation: Essential for developing, calibrating, and validating sensitive analytical methods using HPLC, LC-MS/MS, and GC-MS instrumentation.
• Quality Control in Pharmaceutical Manufacturing: Employed in impurity profiling and ensuring the specificity of assays for drug substance and product quality control.

Basic Information

Product Name	4-Hydroxy Triazolam B-D-Glucuronide
CAS No.	76960-93-5
Molecular Formula	C25H25ClN6O8
Molecular Weight	572.96 g/mol
Synonyms	α-Hydroxytriazolam Glucuronide; 1-Methyl-6-(2-chlorophenyl)-8-hydroxy-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine β-D-Glucopyranosiduronic Acid; 4-Hydroxytriazolam Glucuronide; Triazolam Metabolite Glucuronide; 8-Chloro-6-(2-chlorophenyl)-1-methyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine-4-ol β-D-Glucuronide; UGT Metabolite of Triazolam
EINECS	Contact for details

Quality Control

Our 4-Hydroxy Triazolam B-D-Glucuronide is manufactured under strict quality systems suitable for analytical reference material production. Each batch undergoes rigorous identity confirmation, purity analysis, and stability testing. Comprehensive Certificates of Analysis (COA) detailing HPLC purity, related substance profiles, and spectroscopic data (NMR, MS) are provided to ensure traceability and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum conforms to structure
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.