Description

2-Iminobiotin Hydrazide Hydrochloride is a chemically modified derivative of biotin, designed for advanced biochemical research and pharmaceutical development. This compound is valued for its role as a versatile intermediate and a high-affinity ligand in affinity chromatography and protein purification systems. It is primarily utilized by researchers and manufacturers in the life sciences sector, particularly those focused on enzymology, molecular biology, and the development of novel diagnostic or therapeutic agents targeting biotin-dependent pathways.

Application

• Affinity Chromatography Ligand: Immobilized onto solid supports for the selective purification of avidin, streptavidin, and related proteins.
• Biochemical Research Reagent: Used in studies of biotin-binding proteins, enzyme inhibition assays, and metabolic pathway analysis.
• Pharmaceutical Intermediate: Serves as a key synthetic building block in the development of targeted drug conjugates and radiopharmaceuticals.
• Diagnostic Kit Component: Incorporated into assay systems for the detection and quantification of biotinylated molecules in ELISA and other immunoassays.
• Proteomics and Protein Labeling: Facilitates site-specific labeling and pull-down experiments for protein interaction studies.
• Enzyme Inhibitor Studies: Acts as a research tool to investigate the mechanism of biotin-dependent carboxylases.

Basic Information

Item	Details
Product Name	2-Iminobiotin Hydrazide Hydrochloride
CAS No.	76939-69-0
Molecular Formula	C10H19N5O2S • HCl
Molecular Weight	309.82 g/mol (Free base: 273.35 g/mol)
Synonyms	2-Iminobiotin Hydrazide HCl; Biotin Hydrazide, 2-Imino-; 2-Iminobiotin Hydrazide Monohydrochloride; D-Biotin, 2-imino-, hydrazide, monohydrochloride; 5-[(3aS,4S,6aR)-2-Imino-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid hydrazide hydrochloride; N-(5-[(3aS,4S,6aR)-2-Iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl)hydrazine hydrochloride
EINECS	Contact for details

Quality Control

Our 2-Iminobiotin Hydrazide Hydrochloride is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and heavy metals. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.