Description

2-Iminobiotin n-Hydroxysuccinimide Ester is a high-purity, activated biotin derivative designed for efficient bioconjugation and labeling applications. This compound is critical for researchers and manufacturers requiring reliable, site-specific attachment of biotin tags to primary amines in proteins, peptides, and other biomolecules. It is essential for the development of diagnostic assays, affinity purification systems, and advanced proteomics research in the pharmaceutical and biotechnology sectors.

Application

• Protein and Antibody Biotinylation: Site-specific labeling of lysine residues for use in ELISA, Western blotting, and flow cytometry.
• Affinity Chromatography Probe Synthesis: Preparation of biotinylated ligands for immobilization on streptavidin/avidin resins.
• Diagnostic Kit Development: Conjugation to detection antibodies or antigens in lateral flow assays and immunoassays.
• Cell Surface Labeling: Tagging membrane proteins for visualization and isolation studies.
• Nucleic Acid Probe Labeling: Modification of amine-modified oligonucleotides for detection and capture applications.
• Drug Delivery & Targeting: Conjugation to targeting moieties in the development of biotin-streptavidin based delivery systems.

Basic Information

Product Name	2-Iminobiotin n-Hydroxysuccinimide Ester
CAS No.	76939-67-8
Molecular Formula	C14H20N4O5S
Molecular Weight	356.40 g/mol
Synonyms	2-Iminobiotin NHS Ester; 2-Iminobiotin N-Hydroxysuccinimide Ester; (+)-Biotin N-Succinimidyl Ester; D-Biotin N-Succinimidyl Ester; Biotin-XX, SE; Biotinamidocaproate N-hydroxysuccinimide ester; NHS-Biotin; Succinimidyl Biotinylate
EINECS	Contact for details

Quality Control

Every batch of 2-Iminobiotin n-Hydroxysuccinimide Ester is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with relevant standards for research and in-vitro diagnostic use. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profiles by HPLC and NMR is supplied with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.