Description

H-Ala-Pro-4Mβna · Hcl CAS NO 76496-07-6 is a specialized peptide-derived chromogenic substrate, specifically an alanyl-prolyl-4-methoxy-2-naphthylamide hydrochloride salt. This compound is a critical biochemical tool used as a sensitive and specific substrate for enzymatic activity assays. It is primarily required by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in protease research and enzyme kinetics studies. Its high purity and specificity make it essential for reliable and reproducible experimental results in biochemical and pharmacological applications.

Application

• Protease/Peptidase Research: As a specific fluorogenic substrate for the detection and quantification of dipeptidyl peptidase (DPP) activity, particularly DPP-IV and related enzymes.
• Biochemical Assay Development: Used in the development of high-throughput screening (HTS) assays for drug discovery targeting protease enzymes.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical research into conditions linked to altered peptidase activity.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for proteolytic enzymes in academic and industrial research.
• Cell Biology & Immunology: Used in assays to study the role of specific proteases in cellular processes, immune cell function, and signaling pathways.
• Pharmaceutical Quality Control: Acts as a reference standard or assay substrate in the QC testing of biopharmaceuticals that involve protease activity.

Basic Information

Item	Detail
Product Name	H-Ala-Pro-4Mβna · Hcl
CAS No.	76496-07-6
Molecular Formula	C19H25N3O4 · HCl
Molecular Weight	395.88 g/mol (Free base: 359.42 g/mol)
Synonyms	Ala-Pro-4-Methoxy-2-Naphthylamide Hydrochloride; L-Alanyl-L-Prolyl-4-Methoxy-2-Naphthylamide Hydrochloride; H-Ala-Pro-4-Methoxy-β-NA · HCl; AP-4MβNA · HCl; DPP-IV Substrate (Ala-Pro-AMC analog); 4-Methoxy-2-naphthylamide derivative of Ala-Pro; Fluorogenic Substrate for Dipeptidyl Peptidase
EINECS	Contact for details

Quality Control

Our H-Ala-Pro-4Mβna · Hcl is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to guarantee traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under inert atmosphere to maintain stability and potency. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in DMSO or DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.