Description

β-(4-Azidophenyl)Adenylyl-(3'-5')-Uridine 5'-Diphosphate is a specialized photoactivatable nucleotide analog, commonly known as N6-(4-Azidophenyl)-ADP-ribose or AP-ADPR. This compound is a critical chemical tool for researchers, enabling the covalent cross-linking and labeling of ADP-ribose binding proteins upon UV light exposure. It is primarily utilized in advanced biochemical research for studying ADP-ribosylation signaling pathways, protein-protein interactions, and the development of novel diagnostic assays.

Application

• Photoaffinity Labeling: A key reagent for identifying and characterizing proteins that bind ADP-ribose or its derivatives through UV-induced covalent cross-linking.
• ADP-Ribosylation Research: Used as a substrate or inhibitor analog in enzymatic studies of poly(ADP-ribose) polymerases (PARPs), sirtuins, and other ADP-ribosyltransferases.
• Bioconjugation & Probe Development: Serves as a building block for synthesizing affinity probes, such as biotinylated or fluorescently tagged derivatives, for pull-down assays and microscopy.
• Signal Transduction Studies: Employed to investigate the role of ADP-ribosylation in cellular processes like DNA repair, transcription regulation, and apoptosis.
• Drug Discovery Screening: Utilized in high-throughput assays to identify and evaluate potential inhibitors of ADP-ribosylating enzymes, a target class for cancer and inflammation therapies.

Basic Information

Product Name	β-(4-Azidophenyl)Adenylyl-(3'-5')-Uridine 5'-Diphosphate
CAS No.	76444-18-3
Molecular Formula	C26H28N10O16P2
Molecular Weight	822.51 g/mol
Synonyms	N6-(4-Azidophenyl)-ADP-ribose; AP-ADPR; 4-Azidophenyl-ADP-ribose; N6-(p-Azidophenyl)adenosine diphosphate ribose; 5'-O-[Hydroxy({[hydroxy({[hydroxy(3-hydroxy-3-{[2-({4-azidophenyl}carbamoyl)ethyl]carbamoyl}propoxy)phosphoryl]oxy})phosphoryl]oxy})phosphoryl]uridine; N6-(4-Azidophenyl)-5'-O-{N-[3-(2,3-dihydroxypropanamido)propyl]phosphoramidate}-ADP-ribose (common structural descriptor).
EINECS	Contact for details

Quality Control

Our β-(4-Azidophenyl)Adenylyl-(3'-5')-Uridine 5'-Diphosphate is manufactured under strict quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, spectral data, and stability information is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Due to its hygroscopic and strictly light-sensitive nature, the material should be handled under inert atmosphere or in a controlled, low-humidity environment when aliquoting. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.