Description

25-Hydroxyvitamin D2-25-Glucuronide is a key conjugated metabolite of vitamin D2 (ergocalciferol), formed via glucuronidation. This compound matters significantly as a critical reference standard and analytical tool for accurately quantifying vitamin D2 metabolic pathways in biological samples. It is essential for researchers and manufacturers in the pharmaceutical, clinical diagnostics, and nutritional science industries who require precise measurement of vitamin D status and metabolism.

Application

• Biomedical Research: Used as a high-purity reference standard in studies investigating vitamin D2 metabolism, pharmacokinetics, and biological activity.
• Clinical Diagnostics: Serves as a critical calibrator and control in LC-MS/MS and HPLC assays for the accurate quantification of 25-hydroxyvitamin D2 and its metabolites in serum and plasma.
• Pharmaceutical Development: Employed in the research and quality control of vitamin D-based therapeutics and nutritional supplements to study metabolic fate and ensure product consistency.
• Nutrient Metabolism Studies: A vital tool in nutritional science for understanding the absorption, distribution, and excretion of vitamin D2 in human and animal models.
• Forensic & Doping Control: Applied in specialized analytical laboratories for the detection and confirmation of vitamin D metabolites in forensic toxicology and anti-doping programs.

Basic Information

Product Name	25-Hydroxyvitamin D2-25-Glucuronide
CAS No.	76020-77-4
Molecular Formula	C36H56O9
Molecular Weight	632.83 g/mol
Synonyms	25-Hydroxyergocalciferol-25-Glucuronide; 25(OH)D2-25-Glucuronide; Ergocalciferol Metabolite; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraene-3,25-diol 25-β-D-Glucopyranosiduronic Acid; Vitamin D2 Metabolite; 25-Hydroxyvitamin D2 Glucuronide; Calcidiol D2 Glucuronide
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D2-25-Glucuronide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC and LC-MS, to ensure high chemical purity and structural identity, meeting the exacting standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and identification data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, moisture-free environment. For long-term storage, consider storage under an inert atmosphere. Allow the product to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (HPLC)	≥ 98%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.