Description

n-T-Boc-D-Phe-Ala-Nle p-Nitroanilide is a specialized synthetic peptide derivative containing a chromogenic p-nitroanilide (pNA) group. This compound serves as a critical substrate for the study and characterization of proteolytic enzymes, particularly serine and cysteine proteases. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, biochemistry, and diagnostic assay design to investigate enzyme kinetics, inhibition, and specificity.

Application

• Protease Enzyme Substrate: Used as a chromogenic substrate for the quantitative assay and kinetic analysis of various proteases, including trypsin, chymotrypsin, and elastase.
• Drug Discovery Research: Employed in high-throughput screening (HTS) platforms to identify and evaluate novel protease inhibitors as potential therapeutic agents.
• Biochemical Mechanism Studies: Facilitates research into enzyme reaction mechanisms, specificity, and active site mapping due to its cleavable p-nitroanilide moiety.
• Diagnostic Kit Development: Acts as a key component in the development of colorimetric diagnostic assays for detecting protease activity in clinical or research samples.
• Peptide Chemistry & Synthesis: Serves as an advanced intermediate or model compound in peptide synthesis research and method development.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of protease enzymes used in bioprocessing and manufacturing.

Basic Information

Product Name	n-T-Boc-D-Phe-Ala-Nle p-Nitroanilide
CAS No.	75935-65-8
Molecular Formula	C₃₀H₄₁N₅O₇
Molecular Weight	583.68 g/mol
Synonyms	Boc-D-Phenylalanyl-Alanyl-Norleucine p-Nitroanilide; tert-Butyloxycarbonyl-D-Phenylalanyl-Alanyl-Norleucine 4-Nitroanilide; Boc-D-Phe-Ala-Nle-pNA; N-[(1,1-Dimethylethoxy)carbonyl]-D-phenylalanyl-L-alanyl-L-norleucine 4-nitroanilide; Substrate for Protease Assay; Chromogenic Peptide Substrate; pNA Peptide Derivative
EINECS	Contact for details

Quality Control

Our n-T-Boc-D-Phe-Ala-Nle p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for research and development applications. A detailed Certificate of Analysis (COA) providing batch-specific data for identity, purity, and related substances is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.