Description

Benzyloxycarbonylalanyl-Leucyl-Arginine-2-Naphthylamide is a synthetic tripeptide derivative featuring a chromogenic 2-naphthylamide leaving group. This compound is a critical substrate for the detection and study of proteolytic enzyme activity, particularly for serine and cysteine proteases. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic reagent sectors for enzyme kinetics, inhibitor screening, and assay development.

Application

• Protease Activity Assays: Acts as a chromogenic substrate for quantifying the activity of trypsin-like serine proteases (e.g., thrombin, plasmin, trypsin) and certain cysteine cathepsins.
• Enzyme Kinetic Studies: Used in biochemical research to determine kinetic parameters (Km, Vmax, kcat) and elucidate enzyme mechanisms.
• Inhibitor Screening & Drug Discovery: Serves as a key tool in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Reagent Formulation: Incorporated into in-vitro diagnostic (IVD) kits for clinical measurement of specific protease levels in biological samples.
• Quality Control in Biomanufacturing: Employed to monitor and validate the activity and purity of enzyme preparations during production.
• Cell Biology & Physiology Research: Applied in studies of proteolytic signaling pathways, apoptosis, and other cellular processes involving specific protease activity.

Basic Information

Product Name	Benzyloxycarbonylalanyl-Leucyl-Arginine-2-Naphthylamide
CAS No.	75873-86-8
Molecular Formula	C32H45N7O6
Molecular Weight	623.75 g/mol
Synonyms	Z-Ala-Leu-Arg-2NA; Z-Ala-Leu-Arg-βNA; Cbz-Ala-Leu-Arg-2-Naphthylamide; Benzyloxycarbonyl-Ala-Leu-Arg-2-Naphthylamide; Nα-Carbobenzoxy-L-alanyl-L-leucyl-L-arginine 2-naphthylamide; Protease Substrate Z-Ala-Leu-Arg-2NA; Chromogenic Substrate for Trypsin-like Proteases
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylalanyl-Leucyl-Arginine-2-Naphthylamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and mass spectrometry, and specific optical rotation measurement. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.