Description

4-Hydroxy-3-Nitrophenylacetyl-o-Succinimide Ester is a specialized active ester derivative used as a key reagent in bioconjugation and crosslinking chemistry. Its primary value lies in its ability to efficiently label biomolecules, such as proteins and peptides, under mild aqueous conditions, facilitating the creation of stable conjugates for analytical and diagnostic applications. This compound is essential for researchers and manufacturers in the fields of biotechnology, pharmaceutical development, and in vitro diagnostics, where precise molecular tagging is required.

Application

• Bioconjugation Reagent: For the covalent attachment of ligands, dyes, or other molecules to proteins, antibodies, and peptides.
• Immunoassay Development: Used in the synthesis of enzyme-labeled antibodies or antigens for ELISA and other diagnostic test kits.
• Affinity Chromatography: Immobilization of biomolecules onto solid supports for purification or capture applications.
• Fluorescent Probe Synthesis: Conjugation of fluorophores to targeting molecules for cellular imaging and flow cytometry.
• Drug Delivery Systems: As a linker in the development of antibody-drug conjugates (ADCs) and other targeted therapeutics.
• Protein Crosslinking: For studying protein-protein interactions or stabilizing protein complexes.

Basic Information

Product Name	4-Hydroxy-3-Nitrophenylacetyl-o-Succinimide Ester
CAS No.	75679-31-1
Molecular Formula	C12H10N2O7
Molecular Weight	294.22 g/mol
Synonyms	N-(4-Hydroxy-3-nitrophenylacetyl)succinimide; 2,5-Dioxopyrrolidin-1-yl 2-(4-hydroxy-3-nitrophenyl)acetate; 4-Hydroxy-3-nitrophenylacetic acid N-hydroxysuccinimide ester; 4-Hydroxy-3-nitrophenylacetic acid succinimidyl ester; HNP-Osu; N-Hydroxysuccinimide ester of 4-hydroxy-3-nitrophenylacetic acid; 4-Hydroxy-3-nitrophenylacetyl succinimidyl ester
EINECS	Contact for details

Quality Control

Our 4-Hydroxy-3-Nitrophenylacetyl-o-Succinimide Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our customers' quality system requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.