Description

Suc-Phe-Pro-Phe-Pna CAS NO 75651-68-2 is a synthetic peptide substrate designed for enzymatic activity studies. This compound is of significant value in biochemical research and pharmaceutical development for its role in characterizing protease specificity and kinetics. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme inhibitor screening and proteolytic pathway analysis.

Application

• Protease Substrate: Used as a specific chromogenic or fluorogenic substrate for the study of serine and cysteine proteases, including chymotrypsin and subtilisin.
• Enzyme Kinetics & Inhibition Studies: Essential for determining kinetic parameters (Km, Vmax) and for screening and evaluating potential protease inhibitors in drug discovery.
• Biochemical Assay Development: Incorporated into in-vitro diagnostic (IVD) and research-use-only (RUO) assay kits to detect and quantify specific protease activity.
• Cell Biology Research: Employed in studies of proteolytic signaling cascades, apoptosis, and other cellular processes involving specific peptide cleavage.
• Quality Control in Biologics: Acts as a tool to monitor the presence and activity of host cell proteases during the production and purification of biopharmaceuticals.
• Academic Research: A key reagent in university and institutional labs for fundamental research on protein structure, function, and enzyme mechanisms.

Basic Information

Product Name	Suc-Phe-Pro-Phe-Pna
CAS No.	75651-68-2
Molecular Formula	C35H39N5O8
Molecular Weight	657.72 g/mol
Synonyms	N-Succinyl-Phenylalanyl-Prolyl-Phenylalanyl-p-Nitroanilide; Suc-F-P-F-pNA; Suc-Phe-Pro-Phe-pNA; N-Succinyl-L-Phenylalanyl-L-Prolyl-L-Phenylalanyl 4-Nitroanilide; Chymotrypsin Substrate Suc-FPF-pNA; Protease Substrate 75651-68-2; Suc-FPF-pNA; N-Succinyl-F-P-F-pNA
EINECS	Contact for details

Quality Control

Our Suc-Phe-Pro-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	≥ 95.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.