Description

Sar-Pro-Arg p-Nitroanilide Dihydrochloride is a specialized chromogenic peptide substrate designed for the selective detection and kinetic analysis of serine proteases, particularly thrombin. This compound is critical for biochemical research and diagnostic assay development, offering high sensitivity and specificity in enzymatic activity measurements. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and in-vitro diagnostics (IVD) industries for drug discovery, hemostasis studies, and quality control testing.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric or spectrophotometric determination of thrombin activity in research and diagnostic kits.
• Serine Protease Research: Used in enzymatic studies to investigate the kinetics, inhibition, and specificity of trypsin-like serine proteases.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel anticoagulant and antithrombotic drug candidates.
• IVD Manufacturing: A key raw material in the production of reagents for clinical coagulation analyzers and hemostasis testing panels.
• Biochemical Reagent: Serves as a precise tool in academic and industrial laboratories for protein biochemistry and enzymology experiments.
• Quality Control: Used to standardize and calibrate enzymatic activity in biopharmaceutical production processes involving proteases.

Basic Information

Product Name	Sar-Pro-Arg p-Nitroanilide Dihydrochloride
CAS No.	75241-23-5
Molecular Formula	C22H34Cl2N8O6
Molecular Weight	577.46 g/mol
Synonyms	Suc-Ala-Pro-Arg pNA; Suc-Ala-Pro-Arg-p-nitroanilide dihydrochloride; S-2765 (Chromogenic Substrate); N-Succinyl-Ala-Pro-Arg p-nitroanilide dihydrochloride; Sar-Pro-Arg-pNA·2HCl; Thrombin Chromogenic Substrate S-2765; Suc-APR-pNA; Nα-Succinyl-L-alanyl-L-prolyl-L-arginine 4-nitroanilide dihydrochloride
EINECS	Contact for details

Quality Control

Our Sar-Pro-Arg p-Nitroanilide Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee traceability, consistency, and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spectrometry)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution at 1 mg/mL in water
Functional Activity	Conforms to reference standard in thrombin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.