Description

Fluorescein Glucuronide is a crucial fluorogenic substrate used in enzymatic assays. This compound matters for its high specificity in detecting and quantifying β-glucuronidase activity, a key biomarker in various biochemical and diagnostic applications. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for applications such as reporter gene assays and cell viability testing.

Application

• β-Glucuronidase Reporter Assays: A key substrate for measuring gene expression and promoter activity in molecular biology.
• Diagnostic Kits: Used as a critical component in enzymatic diagnostic kits for detecting bacterial contamination (e.g., E. coli) and certain medical conditions.
• Drug Metabolism Studies: Employed in research to study Phase II drug metabolism via glucuronidation pathways.
• Cell-Based Screening: Utilized in high-throughput screening (HTS) platforms to identify modulators of β-glucuronidase activity for drug discovery.
• Microbiology Research: Applied in assays to detect and enumerate β-glucuronidase-positive microorganisms.
• Biomarker Detection: Facilitates the sensitive detection of β-glucuronidase, which serves as a biomarker for lysosomal storage disorders and other diseases.

Basic Information

Product Name	Fluorescein Glucuronide
CAS No.	74804-84-5
Molecular Formula	C27H28O13
Molecular Weight	560.5 g/mol
Synonyms	Fluorescein Di-β-D-Glucuronide; FDG; Fluorescein Diglucuronide; β-D-Glucopyranosiduronic Acid, 2-[6-(2,7-Dihydroxy-3-oxo-3H-xanthen-9-yl)-2,3-dihydroxybenzoyl]oxy]ethyl, 1-(3-Carboxy-2-hydroxyphenyl) Ester; 9-(2-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one 6-(β-D-Glucopyranosiduronic Acid); GUS Substrate
EINECS	Contact for details

Quality Control

Our Fluorescein Glucuronide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is light-sensitive (store away from light) and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water
Fluorescence Properties	Ex/Em ~490/514 nm (upon enzymatic cleavage)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.