Description

n-(4-(4-Azidophenylazo)Benzoyl)-6-Aminohexyl-N'-Oxysuccinimide Ester CAS NO 74713-45-4 is a heterobifunctional crosslinking reagent featuring a photoactivatable aryl azide group and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is engineered for the efficient, site-specific conjugation of biomolecules, enabling the formation of stable covalent bonds upon UV light activation. It is a critical tool for researchers and manufacturers in bioconjugation, proteomics, and advanced materials science, facilitating the development of diagnostic assays, targeted drug delivery systems, and functionalized surfaces.

Application

• Bioconjugation and Protein Labeling: Covalently linking proteins, antibodies, or peptides to other molecules, surfaces, or solid supports for assay development.
• Photoaffinity Labeling: Identifying and studying protein-ligand interactions and receptor binding sites through UV-induced crosslinking.
• Surface Functionalization: Modifying nanoparticles, biosensor chips, or chromatography media with bioactive ligands for enhanced specificity.
• Polymer and Biomaterial Synthesis: Serving as a crosslinker to create hydrogels or smart polymeric materials with light-responsive properties.
• Diagnostic Kit Manufacturing: Producing stable conjugates for ELISA, lateral flow assays, and other immunodiagnostic platforms.
• Drug Delivery System Development: Constructing antibody-drug conjugates (ADCs) or targeted nanoparticle carriers.

Basic Information

Product Name	n-(4-(4-Azidophenylazo)Benzoyl)-6-Aminohexyl-N'-Oxysuccinimide Ester
CAS No.	74713-45-4
Molecular Formula	C27H25N7O5
Molecular Weight	527.53 g/mol
Synonyms	6-[(4-Azido-2,3,5,6-tetrafluorobenzoyl)amino]hexanoic Acid N-Hydroxysuccinimide Ester (common structural analog name); 4-Azido-2,3,5,6-tetrafluorobenzoic Acid Succinimidyl Ester (related compound); Heterobifunctional Crosslinker, NHS-Azide; Aryl Azide NHS Ester; Photoactivatable NHS Ester; N-Succinimidyl 6-((4-azido-2,3,5,6-tetrafluorobenzoyl)amino)hexanoate; Bioconjugation Reagent 74713-45-4.
EINECS	Contact for details

Quality Control

Our n-(4-(4-Azidophenylazo)Benzoyl)-6-Aminohexyl-N'-Oxysuccinimide Ester is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, identity, and functional suitability are provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.