Description

3-[2-(2-Iodoacetamido)Acetamido]-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is a specialized nitroxide spin label reagent, a critical tool for probing protein structure and dynamics. Its value lies in its ability to form stable covalent bonds with cysteine residues via its iodoacetamide group, enabling precise site-directed spin labeling for Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and manufacturers in structural biology, biophysics, and pharmaceutical development who require high-purity, reliable reagents for studying protein conformation, interactions, and folding mechanisms.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Primary application for covalent attachment to proteins to study structure, dynamics, and conformational changes.
• Protein-Protein Interaction Studies: Used to map binding interfaces and quantify interaction affinities through distance measurements.
• Membrane Protein Research: Essential for labeling and investigating the structure and function of challenging membrane-bound protein targets.
• Drug Discovery & Development: Employed in fragment-based screening and to understand how potential drug molecules affect protein conformation.
• Biophysical Characterization: For measuring distances, mobility, and solvent accessibility within folded proteins and complexes.
• Structural Biology Tool Development: A key reagent in the development of new EPR-based methodologies like DEER (PELDOR) for nanometer-range distance measurements.

Basic Information

Item	Details
Product Name	3-[2-(2-Iodoacetamido)Acetamido]-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy
CAS No.	74648-17-2
Molecular Formula	C14H23IN4O4
Molecular Weight	438.26 g/mol
Synonyms	1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxamidoethyl iodoacetamide; Iodoacetamide-PROXYL; 3-(2-(2-Iodoacetamido)acetamido)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy, free radical; PROXYL-Iodoacetamide; 3-Maleimido-PROXYL derivative (precursor); Nitroxide spin label iodoacetamide; MTSSL precursor analog; EPR spin label reagent for cysteine
EINECS	Contact for details

Quality Control

Every batch of our 3-[2-(2-Iodoacetamido)Acetamido]-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is synthesized and purified under controlled conditions to ensure the highest chemical purity and spin label integrity, critical for sensitive biophysical applications. Our quality assurance includes rigorous analytical testing by HPLC, NMR, and EPR spectroscopy to confirm identity, purity, and radical stability. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	Off-white to pale yellow solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95% (Area %)
Assay (EPR)	≥ 90% active nitroxide radical
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.