Description

5'-O-(4,4'-Dimethoxytrityl)-Thymidine-3'-O-Succinic Acid is a critical phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This compound matters because it serves as a key building block for introducing thymidine nucleotides into DNA sequences with high coupling efficiency and purity. It is essential for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite) for the automated solid-phase synthesis of DNA strands.
• Therapeutic Nucleic Acid Development: Crucial raw material in the production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug discovery.
• Diagnostic Probe Manufacture: Used to synthesize labeled or unlabeled DNA probes for PCR, qPCR, microarrays, and in-situ hybridization.
• Research & Development: Enables academic and industrial labs to create custom DNA sequences for genetic studies, genomics, and molecular biology.
• Primer Production: Key component in the synthesis of high-purity primers for sequencing and amplification techniques.
• Bioconjugation: The succinyl linker provides a reactive handle for further modification and conjugation of oligonucleotides to surfaces or other molecules.

Basic Information

Item	Detail
Product Name	5'-O-(4,4'-Dimethoxytrityl)-Thymidine-3'-O-Succinic Acid
CAS No.	74405-40-6
Molecular Formula	C37H38N2O11
Molecular Weight	686.71 g/mol
Synonyms	DMT-dT-Succinic Acid; 5'-O-DMT-Thymidine-3'-O-Succinate; Thymidine-3'-succinyl-5'-O-(4,4'-dimethoxytrityl); DMT-Thymidine-3'-succinyl CPG support precursor; N-[2-[[(2R,3S,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]oxolan-3-yl]oxy]-2-oxoethyl]succinamic acid (IUPAC); DMT-dT-Su; DMT-T-SUCA
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-Thymidine-3'-O-Succinic Acid is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Products undergo rigorous quality testing, including HPLC for purity and identity verification, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere or with desiccant to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.